Role of inducible nitric oxide synthase in induction of RhoA expression in hearts from diabetic rats

Cardiovascular Research
Hesham SolimanKathleen M MacLeod

Abstract

Recent studies from our laboratory demonstrated that increased expression of the small GTP-binding protein RhoA and activation of the RhoA/rho kinase (ROCK) pathway play an important role in the contractile dysfunction associated with diabetic cardiomyopathy in hearts from streptozotocin (STZ)-induced diabetic rats. Nitric oxide (NO) has been reported to be a positive regulator of RhoA expression in vascular smooth muscle, and we have previously found that the expression of inducible NO synthase (iNOS) is increased in hearts from STZ-diabetic rats. Therefore, in this study, we investigated the hypothesis that induction of iNOS positively regulates RhoA expression in diabetic rat hearts. To determine whether NO and iNOS could increase RhoA expression in the heart, cardiomyocytes from non-diabetic rats were cultured in the presence of the NO donor sodium nitroprusside (SNP) or lipopolysaccharide (LPS) in the absence and presence of the selective iNOS inhibitor, N(6)-(1-iminoethyl)-l-lysine dihydrochloride (L-NIL). In a second study, 1 week after induction of diabetes with STZ, rats were treated with L-NIL (3 mg/kg/day) for 8 more weeks to determine the effect of iNOS inhibition in vivo on RhoA expression and cardiac contractile f...Continue Reading

References

Nov 11, 1994·Journal of Medicinal Chemistry·W M MooreM G Currie
Jan 1, 1995·Advances in Pharmacology·J M GriscavageL J Ignarro
Jan 1, 1997·Annual Review of Immunology·J MacMickingC Nathan
Jan 12, 2001·Physiological Reviews·Y TakaiT Matozaki
Mar 29, 2001·Current Hypertension Reports·K ChitaleyR C Webb
Mar 8, 2002·Hypertension·Kanchan Chitaley, R Clinton Webb
Apr 9, 2002·European Journal of Pharmacology·Thomas M MillsR Clinton Webb
Apr 10, 2002·Circulation·Heinrich TaegtmeyerMartin E Young
Apr 17, 2002·Circulation·Martin E YoungHeinrich Taegtmeyer
Aug 15, 2002·Cardiovascular Research·Naohiko KobayashiHiroaki Matsuoka
Jan 14, 2003·The Journal of Biological Chemistry·Vincent SauzeauPierre Pacaud
Mar 26, 2003·The Journal of Biological Chemistry·Shawn M EllerbroekKeith Burridge
Aug 30, 2003·Circulation Research·Vincent SauzeauPierre Pacaud
Feb 12, 2005·The Journal of Biological Chemistry·Rosalinda MadonnaYong-Jian Geng
Jun 4, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·C A GunnettD D Heistad
Jul 12, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Prabhakara Reddy NagareddyKathleen M MacLeod
Sep 3, 2005·European Journal of Pharmacology·Tina GrönholmIlkka Tikkanen
Dec 27, 2005·Trends in Pharmacological Sciences·Klaudia BudzynChristopher G Sobey
Feb 14, 2006·American Journal of Physiology. Cell Physiology·Kensuke NomaJames K Liao
Feb 18, 2006·Circulation Research·Gervaise LoirandPierre Pacaud
Jul 15, 2006·Trends in Cardiovascular Medicine·Gervaise LoirandPierre Pacaud
Sep 27, 2006·Handbook of Experimental Pharmacology·S Moncada, E A Higgs
Apr 13, 2007·Cardiovascular Research·Guorong LinKathleen M MacLeod
Jul 25, 2007·Journal of Internal Medicine·P-A Jansson
Jul 27, 2007·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Martin M HartgeUlrich Kintscher

❮ Previous
Next ❯

Citations

Jan 31, 2013·Inflammation·Jing TongBingyuan Wang
May 4, 2013·Journal of Bioenergetics and Biomembranes·Nihal OzturkSemir Ozdemir
Dec 31, 2010·Molecular Neurobiology·Bernardo Moreno-LópezDavid González-Forero
Jul 12, 2011·Acta Pharmacologica Sinica·Hong ZhouRui-ying Wang
May 11, 2011·Clinical and Experimental Pharmacology & Physiology·Hiromi JoToshiji Iwasaka
Aug 7, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Hesham SolimanKathleen M MacLeod
Sep 6, 2011·American Journal of Physiology. Heart and Circulatory Physiology·K M ThuetA H Stephenson
Sep 26, 2013·Cardiovascular Diabetology·James T PearsonMikiyasu Shirai
Mar 18, 2015·Pharmacology & Therapeutics·Lucy MurfittAshraf Kitmitto
Mar 1, 2011·Microvascular Research·Caroline C McGownZoë L S Brookes
Apr 7, 2009·European Journal of Pharmacology·Prabhakara Reddy NagareddyKathleen M MacLeod
Feb 11, 2011·Diabetes/metabolism Research and Reviews·Hong Zhou, Yongjun Li
Mar 19, 2014·Journal of Molecular and Cellular Cardiology·Jillian N SimonRicardo Carnicer
Oct 12, 2016·BMC Cardiovascular Disorders·Zhijun SunHongwei Li
Aug 20, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Marion GayardMalvyne Rolli-Derkinderen
Sep 25, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Samantha Souza PossaIolanda de Fátima Lopes Calvo Tibério
Aug 24, 2010·Molecular and Cellular Biochemistry·Christos KalofoutisConstantinos Pantos
Sep 1, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Dongwu LaiGuosheng Fu
Jun 2, 2017·Molecular and Cellular Biochemistry·Aleksandra JovanovicEsma R Isenovic
Aug 28, 2021·Cells·Maria Luísa da Silveira Hahmeyer, José Eduardo da Silva-Santos

❮ Previous
Next ❯

Related Concepts