Role of Inhibitors of Apoptosis Proteins in Testicular Function and Male Fertility: Effects of Polydeoxyribonucleotide Administration in Experimental Varicocele

BioMed Research International
Letteria MinutoliHerbert Marini

Abstract

Neuronal apoptosis inhibitory protein (NAIP) and survivin might play an important role in testicular function. We investigated the effect of PDRN, an agonist of adenosine A2A receptor, on testicular NAIP and survivin expression in an experimental model of varicocele. After the creation of experimental varicocele (28 days), adolescent male Sprague-Dawley rats were randomized to one of the following treatments lasting 21 days: vehicle, PDRN (8 mg/kg i.p., daily), PDRN + 3,7-dimethyl-propargylxanthine (DMPX, a specific adenosine A2A-receptor antagonist, 0.1 mg/kg i.p., daily), varicocelectomy, and varicocelectomy + PDRN (8 mg/kg i.p., daily). Sham-operated animals were used as controls. Animals were then euthanized and testis expression of NAIP and survivin was evaluated through qRT-PCR, western blot, and immunohistochemical analysis. Spermatogenetic activity was also assessed. NAIP and survivin expressions were significantly reduced following varicocele induction when compared to sham animals whereas PDRN-treated rats showed an increase in NAIP and survivin levels. Immunohistochemistry revealed an enhanced expression of NAIP and survivin with a characteristic pattern of cellular localization following PDRN treatment. Moreover, ad...Continue Reading

References

Mar 26, 2003·Cell Structure and Function·Kazuhiro Sakamaki
Sep 16, 2005·European Journal of Pediatric Surgery : Official Journal of Austrian Association of Pediatric Surgery ... [et Al] = Zeitschrift Für Kinderchirurgie·I SukhotnikJ G Mogilner
Aug 12, 2006·Pharmacology, Biochemistry, and Behavior·Cheryl M McCormick, Iva Z Mathews
Sep 16, 2006·Cellular & Molecular Biology Letters·Indrajit ChowdhuryGanapathy K Bhat
May 19, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Johannes K X MaierAlex E MacKenzie
Feb 26, 2008·BJU International·Yuichi SakamotoMasato Fujisawa
Mar 5, 2008·Psychopharmacology·Susanna PietropaoloBenjamin K Yee
Mar 20, 2008·Expert Opinion on Therapeutic Targets·Marzia PennatiNadia Zaffaroni
Jan 16, 2009·World Journal of Urology·Jane-Dar LeeShaw-Yeu Jeng
Oct 29, 2009·Cardiovascular & Hematological Agents in Medicinal Chemistry·Domenica AltavillaFrancesco Squadrito
Mar 5, 2011·Biochemical and Biophysical Research Communications·Sarvenaz KarimpourMohammad-Hossein Ghahremani
Jul 1, 2011·Indian Journal of Urology : IJU : Journal of the Urological Society of India·Michael L Eisenberg, Larry I Lipshultz
Jul 16, 2011·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·May Al-MaghrebiJehoram T Anim
Mar 16, 2012·International Journal of Urology : Official Journal of the Japanese Urological Association·Koji ShiraishiHiroshi Takihara
Oct 12, 2012·Oxidative Medicine and Cellular Longevity·Juan G ReyesRicardo D Moreno
Dec 22, 2012·Andrology·E C SerefogluW J Hellstrom
Mar 22, 2013·Human Reproduction Update·A C J KroeseJ L H Evers
Dec 19, 2013·TheScientificWorldJournal·Giuseppe Santoro, Carmelo Romeo
Feb 14, 2014·Current Urology Reports·No Kwak, David Siegel
Apr 11, 2014·Seminars in Cell & Developmental Biology·Yann Estornes, Mathieu J M Bertrand
May 30, 2014·The World Journal of Men's Health·Ashok AgarwalStefan S du Plessis

❮ Previous
Next ❯

Citations

Jul 26, 2017·Andrologia·S Arena, C Romeo
Jul 7, 2018·Andrology·A M HassaninA N Kaddah
Dec 21, 2018·Andrologia·María Susana Theas
Sep 20, 2018·Oxidative Medicine and Cellular Longevity·Herbert R MariniLetteria Minutoli
Jun 2, 2017·Experimental and Therapeutic Medicine·Salvatore ArenaCarmelo Romeo
Mar 25, 2017·Biology of Reproduction·Jun YinYu-Qi Gao
May 12, 2017·Frontiers in Pharmacology·Francesco SquadritoDomenica Altavilla
Sep 10, 2020·British Journal of Pharmacology·Daolai ZhangZhigang Xu
Feb 3, 2021·International Journal of Molecular Sciences·Pietro AntonuccioLetteria Minutoli

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay
protein assay

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis