PMID: 8612625Mar 1, 1996Paper

Role of insulin-like growth factors in autocrine growth of human retinoblastoma Y79 cells

European Journal of Biochemistry
M GiulianoG Tesoriere

Abstract

In this study, we have demonstrated that human retinoblastoma Y79 cells produce insulin-like growth factors (IGFs) type I and type II and release them into the medium. We have also ascertained, by means of competitive studies and cross-linking procedure, that Y79 cells contain the type-I IGF receptor (IGF-IR). Furthermore, surface-bound IGF-I is internalised by the receptor, then degraded to amino acids. Insulin, IGF-I and IGF-II caused down-regulation of IGF-IR; the effect is concentration and time dependent. Scatchard analysis demonstrated that incubation with insulin markedly decreased the binding capacity measured for IGF-I while the apparent Kd value calculated for IGF-I binding was not significantly modified. IGF-I, IGF-II and insulin induced tyrosine phosphorylation of IGF-IR. Tyrosine phosphorylation of this receptor with, however, a less strong signal, was detectable even in cells cultured in serum-free medium without the addition of any exogenous growth factor. Similar results have been found concerning the tyrosine phosphorylation of insulin receptor substrate-1 (IRS 1). Tyrosine phosphorylation of both IGF-IR and IRS 1, either under basal conditions or after stimulation with growth factors, was strongly inhibited wh...Continue Reading

References

Sep 24, 1992·Nature·T JacksR A Weinberg
Sep 24, 1992·Nature·A R ClarkeH te Riele
Aug 1, 1992·Trends in Biochemical Sciences·D CobrinikR A Weinberg
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J Y ShewW H Lee
Dec 20, 1990·The Journal of Steroid Biochemistry and Molecular Biology·C K OsborneC L Arteaga
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J M HorowitzR A Weinberg
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S H FriendR A Weinberg
Jul 1, 1986·Journal of Neurochemistry·G A SaviolakisG J Chader
Nov 1, 1985·Journal of Neurochemistry·M A YorekB H Ginsberg
Apr 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·A G Knudson
Feb 2, 1984·Nature·A P KyritsisG J Chader
Jul 1, 1995·Neurochemical Research·G TesoriereM Lauricella
Aug 1, 1994·Experimental Eye Research·R VentoM Carabillo
Aug 1, 1994·Nature Genetics·B O WilliamsT Jacks
Jan 1, 1994·Urologia Internationalis·J M WolffF K Habib
Feb 1, 1994·Current Opinion in Genetics & Development·P W Hinds, R A Weinberg
Oct 20, 1993·Regulatory Peptides·M A MeghaniE L Feldman
Oct 20, 1993·Regulatory Peptides·D M MartinE L Feldman

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Citations

Jan 8, 2009·The Oncologist·Su Young KimLee J Helman
Dec 6, 2002·The American Journal of Pathology·Vassiliki PoulakiNicholas Mitsiades
Jan 30, 2013·Journal of Cellular Physiology·Riccardo Di FioreRenza Vento
Nov 18, 2020·Investigational New Drugs·María Belen CancelaPaula Schaiquevich

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