Role of insulin on jejunal PepT1 expression and function regulation in diabetic male and female rats

Canadian Journal of Physiology and Pharmacology
Asdghig H Der-BoghossianAnwar B Bikhazi

Abstract

The aim of this study was to determine whether the jejunal oligopeptide transporter PepT1 is regulated by insulin and whether this regulation is sex-dependent in type 1 diabetic rats. PepT1 expression, real-time polymerase chain reaction, and Western blots were performed using jejunal segments from 4 groups of male and female rats: normal (nondiabetic), insulin-treated nondiabetic, streptozotocin (STZ)-induced diabetic (type 1 diabetes), and insulin-treated diabetic models. Furthermore, the same segments from all groups underwent perfusion to assess uptake of the dipeptide glycylsarcosine through PepT1. Our results showed that insulin treatment of nondiabetic female rats decreased blood glucose level but did not affect nondiabetic male rats. In both male and female diabetic rats, insulin did not completely decrease blood glucose level. Insulin treatment decreased PepT1 mRNA level in nondiabetic male rats and increased mRNA level in nondiabetic female rats without affecting the PepT1 protein level in either sex. Inducing diabetes with STZ increased PepT1 mRNA and protein levels in female rats; however, in diabetic male rats, the increase in mRNA level was accompanied by a decrease in PepT1 protein level. Treatment of diabetic ma...Continue Reading

References

Mar 27, 1996·Biochemical and Biophysical Research Communications·H OgiharaK Takata
Apr 26, 1996·Biochimica Et Biophysica Acta·H SaitoK Inui
Apr 13, 1999·The American Journal of Physiology·M ThamotharanS A Adibi
Sep 25, 1999·Pharmaceutical Research·C Y YangC Pidgeon
Sep 27, 2000·International Journal of Cancer. Journal International Du Cancer·T NakanishiA Tsuji
Nov 6, 2001·Journal of the National Cancer Institute. Monographs·V H Lee
Mar 28, 2003·Journal of Pharmaceutical Sciences·Dea Herrera-Ruiz, Gregory T Knipp
Jun 29, 2005·Molecular Pharmaceutics·Anwar B BikhaziDanielle Jacques
Sep 28, 2005·Diabetes·George L Kellett, Edith Brot-Laroche
Jul 25, 2006·Gender Medicine·Marianne J LegatoUNKNOWN Writing Group for The Partnership for Gender-Specific Medicine

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