Role of insulin receptor in the regulation of glucose uptake in neonatal hepatocytes

Endocrinology
Carmen NevadoM Benito

Abstract

The liver plays a major role in the regulation of glucose homoeostasis. Evidence from liver-specific insulin receptor knockout mice (LIRKO) suggested that insulin's direct and indirect effects on glucose utilization by the liver both require the presence of hepatic insulin receptors (IR). To address this issue, we have generated immortalized neonatal hepatocytes bearing (HIR LoxP) or not (HIR KO) IR. The lack of IR significantly decreased basal glucose uptake in neonatal hepatocytes from 3- to 14-d-old mice, and the expression of glucose transporter 1 (GLUT1), GLUT2, and glucokinase (GK) remained unchanged throughout development. HIR KO reconstituted hepatocytes with IRA but not with IRB isoform and restored basal glucose uptake up to the levels observed in HIR LoxP cells. However, both IR isoforms associated with GLUT1 or GLUT2. Overexpression of IGF-I receptor (IGF-IR) increased basal glucose uptake in neonatal hepatocytes lacking or not IR. This effect was also accompanied by its association with GLUT1 or GLUT2. Exogenous expression of GLUT4 had no effect on basal glucose uptake in neonatal hepatocytes. However, HIR LoxP hepatocytes expressing exogenous GLUT4 increased glucose uptake in the presence of insulin without showin...Continue Reading

Citations

Dec 15, 2012·World Journal of Gastroenterology : WJG·Sumera KarimPatricia F Lalor
Jun 26, 2009·Cellular and Molecular Life Sciences : CMLS·Robert Root-Bernstein, Jessica Vonck
Mar 16, 2011·Archives of Physiology and Biochemistry·Angela M Valverde, Agueda González-Rodríguez
May 10, 2008·Archives of Physiology and Biochemistry·Francesco FrascaRiccardo Vigneri
Jul 23, 2013·Homeopathy : the Journal of the Faculty of Homeopathy·Sathish SampathBalasubramanian Karundevi
Feb 3, 2016·Journal of Ayurveda and Integrative Medicine·Kottireddy SatyanarayanaJayaraman Selvaraj
May 10, 2015·European Journal of Pharmacology·Akilavalli NarasimhanBalasubramanian Karundevi
Sep 13, 2017·International Journal of Obesity : Journal of the International Association for the Study of Obesity·L M LaiglesiaM J Moreno-Aliaga
Dec 5, 2017·Journal of Diabetes Research·O EscribanoA Gómez-Hernández
Aug 27, 2016·Disease Models & Mechanisms·Sabela Diaz-CastroverdeManuel Benito
Jun 7, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Agueda González-RodriguezAngela M Valverde
Oct 7, 2010·American Journal of Physiology. Endocrinology and Metabolism·Gila MaorEddy Karnieli
Sep 4, 2018·Frontiers in Physiology·Natalia de Las HerasAlmudena Gómez-Hernández
Dec 26, 2018·Oxidative Medicine and Cellular Longevity·Ching-Ping YangYih-Hsin Chang
Dec 20, 2018·Journal of Applied Toxicology : JAT·Gokulapriya RajagopalBalasubramanian Karundevi
Jul 30, 2019·BioMed Research International·Xiaoqing XuWenyi Kang
Sep 11, 2020·Nature Reviews. Cancer·Emily J Gallagher, Derek LeRoith
Oct 4, 2017·Endocrine Reviews·Antonino BelfioreRiccardo Vigneri
Oct 18, 2007·Current Opinion in Endocrinology, Diabetes, and Obesity

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