Role of integrin subunits in mesenchymal stem cell differentiation and osteoblast maturation on graphitic carbon-coated microstructured surfaces

Biomaterials
Rene Olivares-NavarreteBarbara D Boyan

Abstract

Surface roughness, topography, chemistry, and energy promote osteoblast differentiation and increase osteogenic local factor production in vitro and bone-to-implant contact in vivo, but the mechanisms involved are not well understood. Knockdown of integrin heterodimer alpha2beta1 (α2β1) blocks the osteogenic effects of the surface, suggesting signaling by this integrin homodimer is required. The purpose of the present study was to separate effects of surface chemistry and surface structure on integrin expression by coating smooth or rough titanium (Ti) substrates with graphitic carbon, retaining surface morphology but altering surface chemistry. Ti surfaces (smooth [Ra < 0.4 μm], rough [Ra ≥ 3.4 μm]) were sputter-coated using a magnetron sputtering system with an ultrapure graphite target, producing a graphitic carbon thin film. Human mesenchymal stem cells and MG63 osteoblast-like cells had higher mRNA for integrin subunits α1, α2, αv, and β1 on rough surfaces in comparison to smooth, and integrin αv on graphitic-carbon-coated rough surfaces in comparison to Ti. Osteogenic differentiation was greater on rough surfaces in comparison to smooth, regardless of chemistry. Silencing integrins β1, α1, or α2 decreased osteoblast matur...Continue Reading

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Citations

Dec 27, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Ernesto Beltrán-PartidaJose M Bastidas
Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Qianli HuangQingling Feng
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May 16, 2019·Journal of Cellular Biochemistry·Helena Bacha LopesMarcio Mateus Beloti
Aug 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaojing HeBin Tang
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Sep 9, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shuying HuNing Gu

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