PMID: 2121884Dec 1, 1990Paper

Role of interferon-gamma in counteracting the suppressive effects of transforming growth factor-beta 2 and glucocorticoids on the production of tumor necrosis factor-alpha

Journal of Leukocyte Biology
D M DunhamK W Kelley

Abstract

The multipotential cytokine, transforming growth factor-beta 2 (TGF-beta 2), is as effective as glucocorticoids in suppressing the production of tumor necrosis factor-alpha (TNF-alpha) by lipopolysaccharide (LPS)-stimulated macrophages, and this inhibition can be abrogated by exogenous interferon-gamma (IFN-gamma). Porcine alveolar macrophages triggered with LPS produce TNF-alpha as identified by complete blocking of cytotoxicity on WEHI 164 clone 13 cells in macrophage supernatants by a monoclonal antibody to human TNF-alpha. Platelet-derived porcine TGF-beta 2, at a concentration of 4 nM, inhibited LPS-induced production of TNF-alpha by 93%. Dexamethasone was as effective as TGF-beta 2, suppressing TNF-alpha production by 86% at a concentration of 4 nM. The natural but less potent glucocorticoid cortisol inhibited TNF-alpha production by 100% at a 100-fold higher concentration (400 nM). Recombinant PoIFN-gamma consistently primed LPS-triggered macrophages for increased production of TNF-alpha by 50-100%, and this priming was totally blocked by a polyclonal antibody to rPoIFN-gamma. Furthermore, the suppression in LPS-induced production of TNF-alpha caused by TGF-beta 2, dexamethasone, and cortisol could be reversed by additio...Continue Reading

Citations

Aug 1, 1993·Immunopharmacology and Immunotoxicology·L SantambrogioG J Thorbecke
Feb 1, 1993·American Journal of Respiratory Cell and Molecular Biology·S K Leeper-WoodfordA A Fowler
Sep 27, 2012·Parasite Immunology·S Nylén, L Eidsmo
Aug 15, 2020·Journal of Leukocyte Biology·Liqun TuYongli Yu
Apr 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·R ScamurraM P Murtaugh
Apr 1, 1993·Clinical and Experimental Immunology·J MaedaK Higashino
Jul 18, 2000·Cellular Immunology·P Méndez-SamperioE García-Martínez
Sep 1, 1993·Baillière's Clinical Obstetrics and Gynaecology·M D MitchellD J Dudley

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