Role of interleukin-1 in induction of matrix metalloproteinases synthesized by rat temporomandibular joint chondrocytes and disc cells

European Journal of Oral Sciences
Y IjimaE Kubota

Abstract

To identify cartilage-degrading enzymes and cell types that can be specifically induced by interleukin-1 (IL-1)alpha, we studied matrix metalloproteinase (MMP) activities of cultured rat temporomandibular joint (TMJ) chondrocytes and disc cells. The cells were isolated from TMJs pre-injected with normal physiological saline (CR) or recombinant human IL-1alpha (AR). MMP activities in the conditioned media were assayed by gelatin enzymography, and they were identified by Western blot analyses. MMP mRNAs in these cells were also detected by RT-PCR. IL-1alpha significantly induced an increase of active MMP9 as well as pro- and active MMP3, but had no effect on the MMP2 activity in both types of cells. MMP3 and MMP9 mRNAs were also inducible in these cells by IL-1alpha stimulation. Furthermore, disc cells were more susceptible to IL-1alpha than chondrocytes. AR cells spontaneously produced the same MMPs in vitro as the CR cells synthesized under IL-1alpha stimulation. The results indicate that MMP9 and MMP3 were predominantly produced by disc cells, and these may be considered to play a pivotal role in ECM degradation during pathological conditions of the TMJ, such as IL-1-induced TMJ arthritis.

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Citations

Sep 28, 2005·Histochemistry and Cell Biology·Naoko ToriyaItaru Mizoguchi
Dec 22, 2004·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·P TiilikainenA Raustia
Oct 1, 2013·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Dawei XuZhiming Cui
Oct 22, 2011·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Michael J GunsonStephen B Milam
May 17, 2008·Journal of Oral Rehabilitation·C Mejersjö, B Wenneberg
Jan 27, 2006·International Journal of Experimental Pathology·Christine L Le MaitreJudith A Hoyland
Mar 15, 2006·Toxicon : Official Journal of the International Society on Toxinology·Cristina Maria FernandesCatarina de Fátima Pereira Teixeira
Jul 20, 2007·Biochemical Society Transactions·C L Le MaitreJ A Hoyland

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