Role of interleukin-1beta in the pathogenesis of diabetic retinopathy

The British Journal of Ophthalmology
Renu A Kowluru, Sarah Odenbach

Abstract

To examine the role of cytokine interleukin-1beta (IL-1beta) in retinal capillary cell death in diabetes. The effect of glucose on the expression of IL-1beta was measured in the bovine retinal endothelial cells. The role of IL-1beta in the accelerated endothelial cell loss was determined by investigating the effect of human recombinant IL-1beta on their apoptosis in normal and high glucose conditions, and was confirmed using interleukin-1 receptor antagonist (IL-1ra). High glucose increased IL-1beta expression by 60% compared with cells incubated in 5 mM glucose (p<0.05). Incubation of cells with IL-1beta increased NO levels by about 80% and activated NF-kappaB by 40%. In the same cells apoptosis was increased by 70% and caspase-3 activity was increased by 40%. Supplementation of IL-1beta in 20 mM glucose medium further increased nitric oxide and NF-kappaB, and accelerated apoptosis, and addition of IL-1ra significantly decreased glucose induced abnormalities and apoptosis. IL-1beta accelerates apoptosis of retinal capillary cells via activation of NF-kappaB, and the process is exacerbated in high glucose conditions. These studies suggest a possible role of IL-1beta in the development of retinopathy in diabetes, and offer a pos...Continue Reading

References

Jun 15, 1996·The Journal of Clinical Investigation·M MizutaniM Lorenzi
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·K MiyamotoA P Adamis
Feb 24, 2000·The British Journal of Ophthalmology·W R MeacockM R Stanford
Aug 28, 2001·Journal of Diabetes and Its Complications·T YuukiS Kishi
Mar 20, 2002·International Journal of Experimental Diabetes Research·Juan MoralesJeffrey L Ram
Apr 23, 2003·Brain, Behavior, and Immunity·Nancy Rothwell
Nov 26, 2003·Investigative Ophthalmology & Visual Science·Renu A Kowluru, Saiyeda Noor Abbas

❮ Previous
Next ❯

Citations

Jun 19, 2012·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Kuo-Feng HuaTzu-Hua Wu
Dec 10, 2009·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Mi In RohOh Woong Kwon
Mar 10, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Siv Johnsen-SorianoFrancisco Javier Romero
Mar 31, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Xi ShenQin Jiao
Sep 6, 2008·Japanese Journal of Ophthalmology·Izumi YuasaYukitaka Uji
Jan 1, 2008·Journal of Molecular Histology·Taoufik KhalfaouiAmel Ouertani-Meddeb
Jul 26, 2008·Reviews in Endocrine & Metabolic Disorders·Sally A Madsen-Bouterse, Renu A Kowluru
Jun 1, 2011·Journal of Ocular Biology, Diseases, and Informatics·Renu A KowluruQing Zhong
Mar 1, 2012·Journal of Ocular Biology, Diseases, and Informatics·Ghulam Mohammad, Mohammad Mairaj Siddiquei
Feb 8, 2011·Antioxidants & Redox Signaling·Tiffany Frey, David A Antonetti
Apr 12, 2007·American Journal of Physiology. Endocrinology and Metabolism·Mohan R DasuIshwarlal Jialal
Jul 21, 2007·Experimental Diabetes Research·Renu A Kowluru, Pooi-See Chan
Apr 18, 2007·Nutrition & Metabolism·Renu A Kowluru, Mamta Kanwar
May 11, 2011·Immunotherapy·Wenbo ZhangRuth B Caldwell
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Dana T Graves, Rayyan A Kayal
May 7, 2013·Clinical Lipidology·Julia V BusikGavin E Reid
Nov 13, 2013·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Chongda ChenXi Shen
Apr 2, 2014·Mediators of Inflammation·Dorota RaczyńskaBartosz L Sikorski
Jul 10, 2014·Indian Journal of Ophthalmology·Sundaram Natarajan
Jul 10, 2014·Indian Journal of Ophthalmology·Chunjie Mao, Hua Yan
Feb 11, 2011·Oxidative Medicine and Cellular Longevity·Ying YangJames R Sowers
Aug 22, 2008·Ocular Immunology and Inflammation·Ermelindo C LealAntónio F Ambrósio
Apr 24, 2012·Expert Opinion on Investigational Drugs·Renu A KowluruJulia M Santos
May 29, 2012·Seminars in Cell & Developmental Biology·Shyamal C BirChristopher G Kevil
Apr 17, 2008·Journal of Diabetes and Its Complications·Carin GustavssonCarl-David Agardh
Dec 19, 2014·Cardiovascular Diabetology·Susana VallejoCarlos F Sánchez-Ferrer
Apr 16, 2015·Mediators of Inflammation·Maria H MadeiraAna R Santiago
Aug 22, 2015·Current Diabetes Reports·Aniruddha AgarwalQuan Dong Nguyen
Nov 11, 2011·Clinical Science·Mohan R DasuRoslyn R Isseroff
Apr 29, 2015·Journal of Diabetes Research·Anna V ZetterqvistMaria F Gomez
Oct 3, 2012·Mediators of Inflammation·Olga Simó-ServatRafael Simó
Sep 27, 2014·Journal of Ophthalmology·Jeffery G GrigsbyAstrid E Cardona
Jul 3, 2015·Journal of Diabetes Research·F SemeraroC Costagliola
May 6, 2015·Progress in Retinal and Eye Research·Rafael Simó, Cristina Hernández

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis