PMID: 2497980Jan 1, 1989Paper

Role of interleukin-2 and interferon-gamma in induction of activated natural killer cells from mice primed in vivo and subsequently challenged in vitro with the streptococcal preparation OK432

Cancer Immunology, Immunotherapy : CII
H YamaueK Saito

Abstract

The natural-killer(NK)-cell-mediated cytotoxicity to syngeneic tumor cells can be augmented by in vivo priming and subsequent in vitro challenge with the streptococcal preparation OK432. Supernatants of cocultures of spleen cells with OK432 contained interleukin-2 (IL-2) and interferon (IFN), mainly IFN-gamma. As the anti-(mouse IFN-gamma) monoclonal antibody but not anti-(mouse IFN-alpha) antibody inhibited the induction of activated NK cells with OK432, the IFN-gamma participated in this response. The enhancement of NK cell activity and production of IL-2 were partially inhibited by the pretreatment of spleen cells with mitomycin C or irradiation, and were completely abolished by pretreatment with actinomycin D. The IL-2 activity after treatment with various metabolic inhibitors ran parallel to the NK activity in a system augmented with OK432. The activity of incubated spleen cells with IL-2 receptors was increased by OK432 treatment, and the NK cell and IFN activities of supernatants were also abrogated by the treatment with anti-(mouse IL-2 receptor) monoclonal antibody, to block the interaction between IL-2 and these receptors of effector cells. The panning method clarified that the incubated spleen cells with IL-2 recepto...Continue Reading

Citations

Jan 1, 1992·Cancer Immunology, Immunotherapy : CII·E KumazawaY Osada
Jun 15, 1989·Immunology Letters·J Bubeník
Jun 1, 1991·Scandinavian Journal of Immunology·M Pinola, E Saksela
Nov 7, 1999·Japanese Journal of Cancer Research : Gann·H TerasawaH Yamaue
Aug 8, 2015·Journal of Neurochemistry·Fengming LanXiao Yue
Mar 16, 1995·International Journal of Cancer. Journal International Du Cancer·M TaniH Terasawa

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