Role of interstitial cells and gap junctions in the transmission of spontaneous Ca2+ signals in detrusor smooth muscles of the guinea-pig urinary bladder

The Journal of Physiology
Hikaru HashitaniHikaru Suzuki

Abstract

To investigate mechanisms underlying the transmission of spontaneous Ca2+ signals in the bladder, changes in intracellular concentrations of Ca2+ ([Ca2+]i) were visualized in isolated detrusor smooth muscle bundles of the guinea-pig urinary bladder loaded with a fluorescent Ca2+ indicator, fura-PE3 or fluo-4. Spontaneous increases in [Ca2+]i (Ca2+ transients) preferentially originated along the boundary of muscle bundles and then spread to the other boundary (Ca2+ waves). The synchronicity of Ca2+ waves across the bundles was disrupted by 18beta-glycyrrhetinic acid (18beta-GA, 40 microm), carbenoxolone (30 microm) or 2-aminoethoxydiphenylborate (2-APB, 50-100 microm), while CPA (10 microm), ryanodine (100 microm), xestospongin C (3 microm) and U-73122 (10 microm) had no effect. Intracellular recordings using two independent microelectrodes demonstrated that 2-APB (100 microm) blocked electrical coupling between detrusor smooth muscle cells. Nifedipine (10 microm) but not nominal Ca2+-free solution diminished the synchronicity of Ca2+ waves before preventing their generation. Staining for c-kit identified interstitial cells (IC) located along both boundaries of muscle bundles. IC were also scattered amongst smooth muscle cells a...Continue Reading

References

Aug 1, 1990·Cell and Tissue Research·G Gabella
Aug 1, 1996·British Journal of Pharmacology·H HashitaniH Suzuki
Jan 14, 1998·Urology·A F Brading
Dec 22, 1999·Microscopy Research and Technique·M HananiS M Faussone-Pellegrini
Apr 15, 2000·The Journal of Physiology·H HashitaniG D Hirst
Jul 15, 2000·The Journal of Physiology·G P SergeantN G McHale
Apr 5, 2001·American Journal of Physiology. Cell Physiology·G P SergeantK D Thornbury
Mar 20, 2002·Experimental Cell Research·Jacques-Antoine HaefligerPaolo Meda
Jul 20, 2002·The Journal of Urology·Karen D McCloskey, Alison M Gurney
Aug 3, 2002·The Journal of Physiology·Satoshi YonedaHikaru Suzuki
Jan 24, 2003·American Journal of Physiology. Renal Physiology·Michael A PezzoneMatthew O Fraser
Feb 11, 2003·The Journal of Physiology·Dirk F van Helden, Mohammad S Imtiaz
Mar 11, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Erik G A HarksAlexander P R Theuvenet
Apr 5, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·George J ChristKarl-Erik Andersson
May 17, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Eniko A SzéllGyula P Szigeti
Jun 10, 2003·The Journal of Physiology·G D S Hirst, S M Ward
Jun 11, 2003·The Journal of Urology·Marcus J DrakeJames I Gillespie

❮ Previous
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Citations

Aug 6, 2008·Der Urologe. Ausg. A·J NeuhausJ U Stolzenburg
Jun 20, 2006·World Journal of Urology·Gouya Ram-LiebigManfred P Wirth
Dec 6, 2006·Pflügers Archiv : European journal of physiology·Takenori MiyashitaMasahiro Sokabe
Jan 19, 2011·Cell and Tissue Research·Aurélie FormeyJean-Jacques Meister
Feb 14, 2006·Nature Clinical Practice. Urology·Alison F Brading, Karen D McCloskey
Aug 25, 2007·International Journal of Impotence Research·A F BradingH Hashitani
Dec 16, 2006·International Journal of Clinical Practice. Supplement·K-E Andersson
Jun 22, 2007·American Journal of Physiology. Renal Physiology·Y IkedaA Kanai
Jan 4, 2008·American Journal of Physiology. Renal Physiology·Louise JohnstonKaren D McCloskey
May 14, 2005·American Journal of Respiratory Cell and Molecular Biology·Neil C FeatherstoneTheodor V Burdyga
Jul 27, 2011·Therapeutic Advances in Urology·Richard Foon, Marcus J Drake
Sep 23, 2010·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Kate E CreedJacqueline K Phillips
Jan 24, 2014·International Journal of MS Care·Gael J YonnetJohn W Rose
Apr 3, 2014·Nephro-urology Monthly·Mohammad Sajjad Rahnama'iPhilip E Van Kerrebroeck
Sep 17, 2014·Nature Reviews. Urology·Bernard T DrummSean M Ward
Oct 9, 2014·Journal of Computational Neuroscience·Shailesh AppukuttanRohit Manchanda
Sep 4, 2008·Expert Opinion on Emerging Drugs·Marcus J Drake
May 16, 2014·BioMed Research International·Kamiel A J KuijpersJack A Schalken
May 26, 2015·Naunyn-Schmiedeberg's Archives of Pharmacology·J I GillespieC Korstanje
May 20, 2015·Naunyn-Schmiedeberg's Archives of Pharmacology·James I GillespieCees Korstanje
Sep 3, 2014·PloS One·Dominika A BijosBahareh Vahabi
Jan 25, 2011·The Journal of Urology·Rebecca M J CunninghamKaren D McCloskey
Mar 28, 2008·The Journal of Nutritional Biochemistry·Jong Hun KimHyong Joo Lee
Feb 18, 2017·International Journal of Molecular Sciences·Beatrice Mihaela RaduSanda Maria Cretoiu
Jan 13, 2006·The Journal of Urology·Magdalini LagouMarcus J Drake
Feb 13, 2007·European Urology·Kamiel A J KuijpersJack A Schalken
Jan 31, 2017·Biochemical and Biophysical Research Communications·Chao WuLongkun Li
Jul 19, 2010·Pharmaceuticals·Isao ArakiMasayuki Takeda
Mar 27, 2015·BJU International·Roger G LentleJohn Paul Chambers
Oct 25, 2011·The Journal of Urology·Michelle LamHikaru Hashitani
May 22, 2010·The Journal of Urology·Louise JohnstonKaren D McCloskey
Jun 19, 2012·The Journal of Urology·Kevin P MonaghanKaren D McCloskey
Apr 23, 2011·The Journal of Urology·Hikaru HashitaniHikaru Suzuki

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