PMID: 9529083Apr 7, 1998Paper

Role of intimin and bundle-forming pili in enteropathogenic Escherichia coli adhesion to pediatric intestinal tissue in vitro

Infection and Immunity
S HicksA D Phillips

Abstract

Attaching and effacing (A/E) lesion formation is central to enteropathogenic Escherichia coli (EPEC) pathogenesis. In vitro experiments with human epithelial cell lines have implicated virulence plasmid-encoded bundle-forming pili (BFP) in initial binding and intimin in intimate attachment and A/E lesion formation. This study investigated the role of BFP and intimin in EPEC interactions with pediatric small intestinal biopsy tissue in in vitro organ culture. Organ culture infections (2 to 8 h) were performed with E2348/69 (a wild-type EPEC O127:H6 clinical isolate) and E2348/69 derivatives including CVD206 (eae deficient), CVD206(pCVD438) (eae-complemented CVD206), CVD206(pCVD438/01) (expressing intimin, which is nonfunctional due to a single amino acid substitution), JPN15 (spontaneous EPEC adherence factor virulence plasmid-cured E2348/69), and 31-6-1(1) (E2348/69 with a TnphoA insertion inactivation mutation in the virulence plasmid-encoded bfpA gene). Scanning and transmission electron microscopy revealed that after 8 h E2348/69 and CVD206 (pCVD438) (both Int+ BFP+) adhered to all specimens, causing A/E lesions with surrounding microvillous elongation. JPN15 and 31-6-1(1) (both Int+ BFP-) adhered and caused A/E lesions alth...Continue Reading

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Citations

Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·V SperandioJ B Kaper
Apr 22, 2003·Infection and Immunity·Nathalie S GonçalvesThomas T MacDonald
Jul 15, 2003·Clinical Microbiology Reviews·S C ClarkeP H Williams
Feb 24, 2004·Infection and Immunity·Hayley J NewtonElizabeth L Hartland
Dec 25, 2004·Infection and Immunity·Alfredo G TorresJames B Kaper
Jan 18, 2005·FEMS Microbiology Reviews·Huiwen Deborah Chen, Gad Frankel
May 19, 2006·Journal of Bacteriology·Cristiano G MoreiraVanessa Sperandio
Jun 20, 2007·Nihon saikingaku zasshi. Japanese journal of bacteriology·Ichiro Tatsuno
Jul 9, 2009·FEMS Immunology and Medical Microbiology·Lucy Beatriz Zapata-QuintanillaSolange Barros Carbonare
Jun 4, 2011·Molecular Nutrition & Food Research·Amy S Van WeyPaul R Shorten
Feb 13, 1999·Molecular Microbiology·G FrankelS Knutton
Sep 7, 2001·Annual Review of Microbiology·B I KazmierczakJ N Engel
Jun 26, 2002·Journal of Pediatric Gastroenterology and Nutrition·Alan D Phillips
May 12, 2006·Journal of Medical Microbiology·Romney M HylandGlen D Armstrong
Feb 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Paul DeanBrendan Kenny
Jul 16, 2002·Infection and Immunity·R J FitzhenryA D Phillips
Dec 19, 2000·Infection and Immunity·C Lindenthal, E A Elsinghorst
Jun 5, 2003·Cellular Microbiology·Jean-Philippe NougayrèdeMichael S Donnenberg
May 11, 2005·Molecular Microbiology·Tracy L Raivio

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