Role of intraluteal prostaglandin F(2alpha), progesterone and oxytocin in basal and pulsatile progesterone release from developing bovine corpus luteum

Prostaglandins & Other Lipid Mediators
Mamadou M BahDariusz J Skarzynski

Abstract

The present study examined the role of intra-luteal prostaglandin (PG) F(2alpha), progesterone (P4) and oxytocin (OT) on the corpus luteum function by using specific hormone antagonists. Luteal cells from the developing CL (days 5-7 of the estrous cycle) were exposed to P4 antagonist (onapristone, OP, 10(-4)M), OT antagonist (atosiban, AT; 10(-6)M) or indomethacin (INDO; 10(-4)M), for 12h and then stimulated with PGF(2alpha) (10(-8)M) for 4h. Pre-treatment of the cells with OP, AT or INDO resulted in an increase in P4 secretion in response to PGF(2alpha). To examine the temporal effects of P4, OT and PGs on P4 secretion, dispersed luteal cells were pre-exposed to OP, AT or INDO for 1, 2, 4, 6 or 12h. Prostaglandin F(2alpha) stimulated P4 secretion (P<0.05) after 2h of pre-exposition. In the microdyalisis study, the spontaneous release of P4 from developing CL tissue was of pulsatile nature with irregular peaks at 1-2h intervals. Treatment with OP increased the number of P4 peaks (P<0.05), whereas AT and INDO significantly reduced the number of P4 peaks detected (P<0.05). Interestingly, INDO completely blocked the pulsatile nature in the release of P4, but it secretion remained stable throughout the experimental period. These re...Continue Reading

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Citations

Apr 1, 2008·Animal Reproduction Science·R M PereiraA E M Horta
Feb 20, 2008·Reproduction in Domestic Animals = Zuchthygiene·A J KorzekwaD J Skarzynski
Aug 24, 2013·Reproduction in Domestic Animals = Zuchthygiene·D J SkarzynskiR Meidan
Feb 22, 2011·Reproduction in Domestic Animals = Zuchthygiene·P C Papa, B Hoffmann
Apr 28, 2020·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Jiongjiong TuFei Gong

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