Role of Isocitrate Dehydrogenase 2 on DNA Hydroxymethylation in Human Airway Smooth Muscle Cells.

American Journal of Respiratory Cell and Molecular Biology
Bonnie H Y YeungWan-Yee Tang

Abstract

Global DNA hydroxymethylation mediated by the TET (ten-eleven translocation) enzyme was induced in allergen-induced airway hyperresponsiveness in mouse lung tissues and specifically in isolated airway smooth muscle (ASM) cells. TET is an α-ketoglutarate (α-KG)-dependent enzyme, and the production of α-KG is catalyzed by IDH (isocitrate dehydrogenase). However, the role of IDH in the regulation of DNA hydroxymethylation in ASM cells is unknown. In comparison with nonasthmatic cells, asthmatic ASM cells exhibited higher TET activity and IDH2 (but not IDH-1 or IDH-3) gene expression levels. We modified the expression of IDH2 in ASM cells from humans with asthma by siRNA and examined the α-KG levels, TET activity, global DNA hydroxymethylation, cell proliferation, and expression of ASM phenotypic genes. Inhibition of IDH2 in asthmatic ASM cells decreased the α-KG levels, TET activity, and global DNA hydroxymethylation, and reversed the aberrant ASM phenotypes (including decreased cell proliferation and ASM phenotypic gene expression). Specifically, asthmatic cells transfected with siRNA against IDH2 showed decreased 5hmC (5-hydroxymethylcytosine) levels at the TGFB2 (transforming growth factor-β2) promoter determined by oxidative b...Continue Reading

References

Oct 7, 2004·The American Journal of Pathology·Hong Wei ChuSally E Wenzel
Feb 28, 2007·Immunology and Cell Biology·Toluwalope MakindeDevendra K Agrawal
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Annie V LeDavid H Broide
Sep 12, 2007·Molecular and Cellular Biology·Suming HuangGary Felsenfeld
Aug 2, 2008·The European Respiratory Journal·S ZuyderduynJ K Burgess
Feb 21, 2009·The New England Journal of Medicine·Hai YanDarell D Bigner
Nov 26, 2009·Nature·Lenny DangShinsan M Su
Jun 2, 2010·Journal of the National Cancer Institute·Zachary J Reitman, Hai Yan
Dec 1, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xianglan YaoStewart J Levine
Feb 12, 2011·The European Respiratory Journal·S BaraldoM Saetta
Apr 5, 2011·EMBO Reports·Rasheduzzaman ChowdhuryAkane Kawamura
May 27, 2011·The New England Journal of Medicine·Christopher L GraingePeter H Howarth
Jun 21, 2011·Immunological Reviews·Cynthia J Koziol-White, Reynold A Panettieri
Dec 14, 2011·Genes & Development·Hao Wu, Yi Zhang
Jan 24, 2012·Molecular and Cellular Endocrinology·Simon G RoyceMimi L K Tang
Mar 6, 2012·The Journal of Allergy and Clinical Immunology·Cara J BossleySejal Saglani
Mar 15, 2012·The Journal of Biological Chemistry·Wei YuJohn M Denu
Jun 13, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rachel L CliffordAlan J Knox
Aug 25, 2012·Human Pathology·Dinesh RakhejaWeina Chen
Oct 17, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Donald M MillerKara Sedoris
Mar 26, 2013·American Journal of Respiratory Cell and Molecular Biology·Yan ShangWan-yee Tang
Sep 7, 2013·Nature Protocols·Michael J BoothShankar Balasubramanian
Jan 22, 2014·Environmental and Molecular Mutagenesis·Robert Ys ChengWan-Yee Tang
Mar 14, 2014·The Lancet. Respiratory Medicine·Michael H ChoUNKNOWN NETT Genetics, ICGN, ECLIPSE and COPDGene Investigators
Jun 10, 2014·Ageing Research Reviews·Antero SalminenKai Kaarniranta
Jun 25, 2015·The Open Respiratory Medicine Journal·L F Keglowich, P Borger
Jul 17, 2015·American Journal of Respiratory Cell and Molecular Biology·Amanda H Y LinWan-yee Tang
Dec 20, 2015·The Journal of Allergy and Clinical Immunology·Hari K SomineniHong Ji
Mar 5, 2016·The Journal of Allergy and Clinical Immunology·Steven S AnStephen B Liggett
Mar 22, 2016·PloS One·Mythili DileepanMathur S Kannan
Jul 18, 2017·Trends in Cell Biology·Laurence M GagnéFrédérick A Mallette
Oct 7, 2017·American Journal of Respiratory Cell and Molecular Biology·Christie A OjiakuReynold A Panettieri
Dec 2, 2017·The European Respiratory Journal·Pieter S Hiemstra, Anne M van der Does
Aug 22, 2018·International Journal of Molecular Sciences·Akira SaitoTakahide Nagase
Oct 26, 2018·Frontiers in Genetics·Vera Miranda-GonçalvesCarmen Jerónimo
Nov 9, 2018·Frontiers in Genetics·Silvia Raineri, Jane Mellor
Feb 12, 2019·American Journal of Respiratory Cell and Molecular Biology·Christie A OjiakuReynold A Panettieri

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Citations

Apr 1, 2020·American Journal of Respiratory Cell and Molecular Biology·Steven K Huang
Jun 29, 2021·American Journal of Respiratory and Critical Care Medicine·Andrew J HalaykoPrescott G Woodruff

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Methods Mentioned

BETA
transfection
Assay
ELISA
PCR
methylation
immunoprecipitation
SMA
acetylation

Software Mentioned

GraphPad
MethPrimer
Prism6

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