Role of Ku70 in the apoptosis of inflamed dental pulp stem cells

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
Yequan HuangLiuyan Meng

Abstract

The study aimed to investigate the effects of DNA repair proteins on cell apoptosis in human DPSCs during inflammation. Lipopolysaccharide (LPS) was used to stimulate inflammation in dental pulp in vivo and in vitro. We identified the activation of DSB response and DNA repair proteins in inflamed pulp tissue and in LPS-treated human DPSCs. Then we transfected the cells with Ku70 (a key protein involved in NHEJ) siRNA and detected the expression changes of γ-H2A.X, DNA repair proteins and cell apoptosis. Immunohistochemical staining showed that at 4 and 6 days of pulpitis the expression of Ku70 and γ-H2A.X significantly increased. The levels of γ-H2A.X, Ku70, Xrcc4, and Rad51 increased considerably in the LPS-treated DPSCs. Furthermore, decreased expression of Ku70 could increase the number of γ-H2A.X foci, apoptotic cells and reduce cell viability in DPSCs. The results indicate that NHEJ pathway was the main mechanism involved in DNA damage response induced by repeated LPS stimulation in DPSCs. Meanwhile, the findings suggested that Ku70 serves importantly in the apoptosis of DPSCs in the inflammatory environment.

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Citations

Apr 7, 2019·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Chinnadurai ManiKomaraiah Palle

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Methods Mentioned

BETA
flow cytometry
PCR
transfect
transfection

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis