PMID: 8986233Dec 1, 1996Paper

Role of Kupffer cell-derived reactive oxygen intermediates in alcoholic liver disease in rats in vivo

Alcoholism, Clinical and Experimental Research
Y TakeyamaM Okumura

Abstract

The pathophysiology of alcoholic liver disease (ALD) remains largely unknown. In this work, we have developed an experimental rat model to elucidate the mechanism of liver injury, including ALD, in which Kupffer cell-derived reactive oxygen intermediates (ROIs) might be involved. Groups of male Wistar rats were pair-fed on a liquid high-fat diet containing ethanol (36% of total calories) or isocaloric carbohydrate with or without dietary carbonyl iron (0.5% w/v) for 3 weeks. In this rat model, we investigated Kupffer cell-derived ROI generation, which affected hepatocellular injury and hepatic fibrosis in ALD. The production of ROIs in Kupffer cells isolated from the iron-fed, the ethanol-fed, and the ethanol plus iron-fed rats were significantly increased, compared with that in Kupffer cells isolated from control rats (iron > ethanol+iron > ethanol > > control). However, hepatic vitamin E content in the ethanol plus iron-fed rats was decreased rather than that in the iron-fed rats. Then, lipid peroxidation of isolated microsomes was assessed as malondialdehyde equivalents determined by thiobarbituric acid assay. Compared with controls, the malondialdehyde equivalents were elevated in experimental groups (ethanol+iron > ethanol...Continue Reading

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Citations

Aug 10, 2001·Journal of Hepatology·H Moshage
Aug 4, 2009·Chemical Research in Toxicology·Debra L Laskin
Oct 24, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Daniel Y HungMichael S Roberts
Jun 15, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Hiroko IijimaShiro Maeyama
May 23, 2002·The Journal of Pharmacology and Experimental Therapeutics·Daniel Y HungMichael S Roberts

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