Role of l-carnitine and oleate in myogenic differentiation: implications for myofiber regeneration

Journal of Exercise Nutrition & Biochemistry
Hojun LeeSeung-Jun Choi

Abstract

Myogenic progenitors play a critical role in injury-induced myofiber regeneration. The purpose of this study was to characterize the effects of oleate and L-carnitine on the overall behavior of proliferating myogenic progenitors (myoblasts) and its link to the mitochondrial biogenic process. C2C12 myoblasts were cultured either with no treatment, oleate, L-carnitine, or their mixture. Proliferating myoblasts were investigated under a phase-contrast microscope. Myonuclei and myosin heavy chain were stained with DAPI and MF20 antibody, respectively, in differentiated myotubes and visualized under florescence microscopy. Mitochondrial biogenic markers and porin were assessed by qRT-PCR or immunoblotting. Increased proliferation rate was observed in myoblasts conditioned with oleate or a mixture of oleate and L-carnitine in contrast to that in non-treated (NT) and L-carnitine-treated myoblasts. Myoblast viability was not statistically different among all tested groups. Fusion index and myotube width were greater in oleate- or L-carnitine-conditioned myotubes than those in NT myotubes, with the greatest effect seen in myotubes conditioned with the mixture. The gene expressions of Pgc1-α, Nrf1, and Tfam were the greatest in myotubes ...Continue Reading

References

Jun 1, 1973·The American Journal of Anatomy·B M Carlson
Apr 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·R Bischoff
Dec 10, 2002·Biochemical and Biophysical Research Communications·Katrin EitelMonika Kellerer
Jan 5, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anne GarnierRenée Ventura-Clapier
Jan 8, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·James G Tidball
Apr 27, 2005·The American Journal of Sports Medicine·Tero A H JärvinenMarkku Järvinen
Jul 5, 2006·Genes & Development·Xiaozhong Shi, Daniel J Garry
Jun 7, 2007·American Journal of Physiology. Endocrinology and Metabolism·Hakam AlkhateebArend Bonen
Jul 21, 2007·Experimental Diabetes Research·Panchamoorthy Rajasekar, Carani Venkatraman Anuradha
Nov 13, 2007·Current Opinion in Cell Biology·Fabien Le Grand, Michael A Rudnicki
Jul 18, 2009·Stem Cells·Ashley L SiegelD D W Cornelison
Aug 22, 2009·Circulation Research·Joon-Young ParkPaul M Hwang
Jan 7, 2010·The Journal of Clinical Investigation·Francesco Saverio TedescoGiulio Cossu
Sep 8, 2010·Cellular and Molecular Life Sciences : CMLS·Pascal P H HommelbergRamon C J Langen
Jun 27, 2012·Exercise and Sport Sciences Reviews·Zhen YanNicholas P Greene
Jan 11, 2013·Physiological Reviews·Hang YinMichael A Rudnicki
Aug 2, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Boa KimJoon-Young Park
Apr 4, 2015·Oxidative Medicine and Cellular Longevity·Anna MontesanoIleana Terruzzi
Dec 23, 2015·Ageing Research Reviews·Carla Domingues-FariaStephane Walrand
Sep 28, 2017·Oxidative Medicine and Cellular Longevity·Hojun LeeSeung-Jun Choi

❮ Previous
Next ❯

Methods Mentioned

BETA
florescence microscopy

Software Mentioned

ImageJ

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.