Role of L-type Ca2+ channels in neural stem/progenitor cell differentiation

The European Journal of Neuroscience
Marcello D'AscenzoClaudio Grassi

Abstract

Ca(2+) influx through voltage-gated Ca(2+) channels, especially the L-type (Ca(v)1), activates downstream signaling to the nucleus that affects gene expression and, consequently, cell fate. We hypothesized that these Ca(2+) signals may also influence the neuronal differentiation of neural stem/progenitor cells (NSCs) derived from the brain cortex of postnatal mice. We first studied Ca(2+) transients induced by membrane depolarization in Fluo 4-AM-loaded NSCs using confocal microscopy. Undifferentiated cells (nestin(+)) exhibited no detectable Ca(2+) signals whereas, during 12 days of fetal bovine serum-induced differentiation, neurons (beta-III-tubulin(+)/MAP2(+)) displayed time-dependent increases in intracellular Ca(2+) transients, with DeltaF/F ratios ranging from 0.4 on day 3 to 3.3 on day 12. Patch-clamp experiments revealed similar correlation between NSC differentiation and macroscopic Ba(2+) current density. These currents were markedly reduced (-77%) by Ca(v)1 channel blockade with 5 microm nifedipine. To determine the influence of Ca(v)1-mediated Ca(2+) influx on NSC differentiation, cells were cultured in differentiative medium with either nifedipine (5 microm) or the L-channel activator Bay K 8644 (10 microm). The l...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L J RichardsP F Bartlett
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Feb 1, 1994·Current Opinion in Neurobiology·N C SpitzerE Olson
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·F H GageJ Ray
Jun 1, 1997·Current Opinion in Neurobiology·H BitoR W Tsien
May 28, 1998·Cell Calcium·P Nicotera, S Orrenius
Oct 28, 1998·Nature·M J BerridgeP Lipp
Apr 7, 1999·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·J Krebs
Oct 3, 1999·Physiological Reviews·A TakahashiB Herman
Feb 26, 2000·Science·F H Gage
Mar 15, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·S ArnholdK Addicks
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·A E WestM E Greenberg
Jan 22, 2003·Trends in Neurosciences·Giles E Hardingham, Hilmar Bading
Jul 10, 2003·Current Opinion in Neurobiology·Karl DeisserothRichard W Tsien
Dec 17, 2003·Journal of Neurochemistry·Jingli CaiKatsutoshi Furukawa
May 14, 2004·Neuroscience Research·V BalasubramaniyanJ C V M Copray
May 26, 2004·Neuron·Karl DeisserothRobert C Malenka
Sep 18, 2004·Trends in Neurosciences·Emil C ToescuPhilip W Landfield
Oct 16, 2004·Journal of Neurophysiology·Diane LipscombeWeifeng Xu

❮ Previous
Next ❯

Citations

Dec 21, 2011·Molecular Genetics and Genomics : MGG·Martin L Rein, Jan M Deussing
Jun 10, 2010·The Journal of Biological Chemistry·Leigh Anne SwayneSteffany A L Bennett
Jan 14, 2011·American Journal of Physiology. Cell Physiology·Petra GeierHelmut Kubista
Aug 29, 2009·Cell Communication and Signaling : CCS·Rodrigo R Resende, Avishek Adhikari
Dec 14, 2006·Journal of Cell Science·Adeline F DeyrieuxVan G Wilson
May 16, 2013·PloS One·Nawal Zabouri, Silke Haverkamp
Nov 4, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Dan ZhangJonathan S Marchant
May 25, 2011·World Journal of Stem Cells·Gui-Rong Li, Xiu-Ling Deng
May 8, 2014·Experimental Cell Research·Silvana PapagerakisPetros Papagerakis
Oct 22, 2013·Pflügers Archiv : European journal of physiology·Maria Vittoria Podda, Claudio Grassi
May 25, 2010·Cellular and Molecular Life Sciences : CMLS·Paola LucianiAlessandro Peri
Jul 29, 2010·Stem Cell Reviews and Reports·Carlos Atico ArizaSurya K Mallapragada
Dec 19, 2006·Journal of Neurochemistry·Kurt F HauserPamela E Knapp
Oct 2, 2012·Stem Cells International·Julieta Aprea, Federico Calegari
May 23, 2012·Channels·Leigh Anne Swayne, Leigh Wicki-Stordeur
Jan 27, 2009·Progress in Histochemistry and Cytochemistry·Richard H W FunkNurdan Ozkucur
Aug 1, 2008·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Chae Gil LimSeung Cheol Ahn
Mar 4, 2010·Biotechnology and Bioengineering·Ze-Zhi WuSteven L Stice
May 25, 2010·Journal of Neurochemistry·Takahiro Yasuda, David J Adams
Jan 15, 2008·The European Journal of Neuroscience·Nidhi Gakhar-KoppoleFrancesca Ciccolini
Apr 23, 2014·Stem Cells·Virve KärkkäinenJari Koistinaho
Oct 7, 2014·Development, Growth & Differentiation·Daniel B L TehHiromu Yawo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.