Role of ldb1 in adult intestinal homeostasis.

International Journal of Biological Sciences
Ipsita Dey-GuhaHeiner Westphal

Abstract

Ldb1 is an essential co-regulator of transcription in embryonic development. It acts in conjunction with nuclear LIM-homeodomain and LIM-only proteins to control key events of organogenesis as precursor cells enter lineage specification. Here we ask whether Ldb1 exerts control over stem cell activation and differentiation throughout the life of the organism as required for tissue homeostasis. To help answer this question, we have generated conditional Ldb1 mouse mutants with an Ldb1 floxed/floxed;ROSA26CreER genotype. Tamoxifen treatment of 60 day-old mutant animals results in near-ubiquitous Cre-mediated Ldb1 inactivation. As a consequence, the stem cell microenvironment of intestinal crypts is drastically affected. Cells that normally express Ldb1 together with markers that identify them as lineage progenitors cease to retain bromodeoxyuridine and are gradually lost. Ldb1 inactivation in intestinal crypts and/or in neighboring mesenchymal cells also triggers activation of Wnt signaling in the stem cell niches of the small intestine. Cell proliferation is markedly increased in the epithelia of the small intestine, and Lgr5-expressing stem cells disappear from the base of the crypts. This perturbation of the normal process of t...Continue Reading

References

Jan 1, 1993·Annual Review of Cell Biology·C Birchmeier, W Birchmeier
Feb 1, 2000·Trends in Genetics : TIG·O Hobert, H Westphal
May 23, 2002·Developmental Cell·Thomas AndlSarah E Millar
Jul 17, 2003·Genes & Development·Daniel PintoHans Clevers
Dec 4, 2003·EMBO Reports·Jacqueline M Matthews, Jane E Visvader
Dec 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Frank KuhnertCalvin J Kuo
Jun 17, 2004·Genes & Development·Owen J SansomDouglas J Winton
Aug 9, 2005·Gastroenterology·Alex GregorieffHans Clevers
May 5, 2006·Development·Mahua MukhopadhyayHeiner Westphal
Jul 1, 2006·Science·Horace RheeElaine Fuchs
Jul 11, 2006·Annual Review of Cell and Developmental Biology·Cédric Blanpain, Elaine Fuchs
Feb 10, 2007·Cell·Cédric BlanpainElaine Fuchs
Feb 27, 2007·Gastroenterology·Laurens G Van der FlierHans Clevers
Aug 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yangu ZhaoHeiner Westphal
Dec 15, 2007·Journal of Cellular Biochemistry·Ipsita Dey-GuhaHeiner Westphal
Jun 10, 2008·Nature Genetics·Eugenio Sangiorgi, Mario R Capecchi
Jul 17, 2008·Genes & Development·Nick BarkerHans Clevers
Dec 6, 2008·BMC Gastroenterology·Paige S DaviesMelissa H Wong

❮ Previous
Next ❯

Citations

Oct 2, 2019·Proceedings of the National Academy of Sciences of the United States of America·Miha RenkoMariann Bienz
Oct 8, 2016·Oncotarget·Sebastián A GarcíaSebastian Schölch

❮ Previous
Next ❯

Software Mentioned

Slidebook
Photoshop
QImaging

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis