Role of lincRNA-Cox2 targeting miR-150 in regulating the viability of chondrocytes in osteoarthritis.

Experimental and Therapeutic Medicine
Meng JiangJianping Cao

Abstract

Osteoarthritis (OA) is a joint disease characterised by progressive cartilage degradation and inflammation, but the detailed pathogenesis of OA remains unclear. The present study aimed to investigate the role of long intergenic non-coding RNA (lincRNA)-Cox2 in OA progression and the potential mechanism. An OA mouse model was used for in vivo experiments, and IL-1β-induced injury of mouse chondrocytes was conducted for in vitro experiments. Small interfering (si)-Cox2 was transfected into chondrocytes to elucidate the effect of lincRNA-Cox2 on OA. Quantitative reverse transcription PCR assays were conducted to detect the expression of lincRNA-Cox2 and microRNA (miR)-150. Cell proliferation and apoptosis were analysed based on an MTT assay and annexin V/propidium iodide staining, respectively. Western blotting was performed to evaluate the protein expression levels of Ki-67, PCNA, Bax, cleaved (c)-Caspase-3, c-Caspase-9 and Wnt/β-catenin pathway-associated proteins in chondrocytes. High levels of lincRNA-Cox2 were observed in cartilage tissues of the OA mouse model in vivo. In the in vitro experiments, the expression of lincRNA-Cox2 was increased in IL-1β-treated chondrocytes. Knockdown of lincRNA-Cox2 promoted the proliferation ...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 6, 2007·Journal of Cellular Physiology·Mary B Goldring, Steven R Goldring
Aug 21, 2008·Nature Protocols·Marjolaine GossetClaire Jacques
Sep 16, 2010·BMC Musculoskeletal Disorders·Takuya NiimotoMitsuo Ochi
Aug 3, 2013·Science·Susan CarpenterKatherine A Fitzgerald
Jan 17, 2014·Nature·Yvonne TayPier Paolo Pandolfi
Aug 26, 2015·Current Drug Targets·Xiaoliang YuanLonghuo Wu
Nov 19, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qiang TongXian-Ming Chen
Jun 2, 2016·Acta histochemica·Miroslava JuríkováIvan Varga
Aug 31, 2016·Frontiers in Microbiology·Parmila VermaVijay K Prajapati
Dec 22, 2016·International Journal of Molecular Sciences·Edith CharlierDominique De Seny
Aug 23, 2017·Nature Immunology·Y Grace ChenHoward Y Chang
Apr 19, 2018·Cell Death and Differentiation·Zhenyi XueRongxin Zhang
Nov 8, 2018·Cell Reports·Roland EllingSusan Carpenter
Feb 28, 2019·Osteoarthritis and Cartilage·M A Jeffries
Jun 6, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Amy L BrownFabricio Passador-Santos
Jul 16, 2019·Cell & Bioscience·Ying ZhangLiu Yang
Nov 23, 2019·Stem Cell Research & Therapy·Jian ZhuChengzhen Liang
Nov 30, 2019·Microbial Pathogenesis·Maryam AghaeiSeyed Hossein Hejazi
Jan 7, 2020·The Journal of Gene Medicine·Pengfei SunYuhua Jia
Jan 12, 2020·Wiley Interdisciplinary Reviews. RNA·Siyu JiangMin Yang
Jan 12, 2020·Journal of Proteomics·M R Eveque-MourrouxB Cillero-Pastor
Jan 29, 2020·Current Opinion in Cell Biology·Jorge Domínguez-AndrésMihai G Netea
Feb 3, 2020·International Immunopharmacology·Toshio Takahashi

❮ Previous
Next ❯

Software Mentioned

ImageJ
CellQuest Pro
DIANA
SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis