Role of Lipid Composition on the Interaction between a Tryptophan-Rich Protein and Model Bacterial Membranes

Langmuir : the ACS Journal of Surfaces and Colloids
Michael SandersRebecca J Green

Abstract

The interaction between tryptophan-rich puroindoline proteins and model bacterial membranes at the air-liquid interface has been investigated by FTIR spectroscopy, surface pressure measurements, and Brewster angle microscopy. The role of different lipid constituents on the interactions between lipid membrane and protein was studied using wild type (Pin-b) and mutant (Trp44 to Arg44 mutant, Pin-bs) puroindoline proteins. The results show differences in the lipid selectivity of the two proteins in terms of preferential binding to specific lipid head groups in mixed lipid systems. Pin-b wild type was able to penetrate mixed layers of phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) head groups more deeply compared to the mutant Pin-bs. Increasing saturation of the lipid tails increased penetration and adsorption of Pin-b wild type, but again the response of the mutant form differed. The results provide insight as to the role of membrane architecture, lipid composition, and fluidity on antimicrobial activity of proteins. Data show distinct differences in the lipid binding behavior of Pin-b as a result of a single residue mutation, highlighting the importance of hydrophobic and charged amino acids in antimicrobial protein...Continue Reading

References

Nov 1, 1993·FEMS Microbiology Reviews·G I VeldW N Konings
May 10, 2002·Plant Molecular Biology·Craig F Morris
Mar 5, 2004·Biotechnology & Genetic Engineering Reviews·Michael J GirouxJohn Sherwood
Oct 22, 2005·Plant Molecular Biology·Rosanna CapparelliMauro Cresti
Nov 8, 2005·Physical Chemistry Chemical Physics : PCCP·Mitaben D LadRebecca J Green
Jun 8, 2006·Biochimica Et Biophysica Acta·David I ChanHans J Vogel
Nov 2, 2006·The FEBS Journal·Li DayJ David Schofield
Jan 2, 2007·Biotechnology Advances·Didier MarionKhalil Elmorjani
Feb 28, 2007·Biophysical Journal·Mitaben D LadRebecca J Green
Sep 30, 2008·Biochimica Et Biophysica Acta·Richard M Epand, Raquel F Epand
Dec 5, 2008·The Journal of Physical Chemistry. B·Luke A CliftonRichard A Frazier
Nov 20, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Xiangke ChenHeather C Allen
Mar 23, 2011·Cold Spring Harbor Perspectives in Biology·Andrew Callan-JonesPatricia Bassereau
Apr 12, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Richard M Epand, Raquel F Epand
Aug 27, 2011·Physical Chemistry Chemical Physics : PCCP·Luke A CliftonRebecca J Green
Nov 3, 2011·Current Protein & Peptide Science·Larisa E Cybulski, Diego de Mendoza
Apr 9, 2013·Biochimica Et Biophysica Acta·Evan F HaneyHans J Vogel
Mar 15, 2015·Biochimica Et Biophysica Acta·Fábio FernandesLuís M S Loura
Jun 4, 2015·Chemistry and Physics of Lipids·Thomas K M Nyholm

❮ Previous
Next ❯

Citations

Apr 28, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Michael R SandersRebecca J Green
May 28, 2019·World Journal of Microbiology & Biotechnology·Craig F Morris
Feb 23, 2020·International Journal of Molecular Sciences·Xiaoyan LiGuangyuan He
Apr 14, 2018·World Journal of Microbiology & Biotechnology·Nadin ShagaghiMrinal Bhave
May 15, 2021·Biochimica Et Biophysica Acta. Biomembranes·Ritika GuptaSajal K Ghosh
Oct 30, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Sara AnselmoValeria Vetri

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.