PMID: 12785523Jun 6, 2003Paper

Role of lipopolysaccharides in the adhesion, retention, and transport of Escherichia coli JM109

Environmental Science & Technology
Nehal I Abu-Lail, Terri A Camesano

Abstract

The role of lipopolysaccharides (LPS) in bacterial adhesion was investigated via atomic force microscopy (AFM). Adhesion between a silicon nitride tip and Escherichia coli JM109 was measured in water and 0.01 M phosphate-buffered saline (PBS) on untreated cells and on a sample of E. coli treated with 100 mM ethylenediaminetetraacetic acid (EDTA), which removes approximately 80% of the LPS molecules. LPS removal decreased the adhesion affinity between the bacterial cells and the AFM tip from -2.1 +/- 1.8 to -0.40 +/- 0.36 nN in water and from -0.74 +/- 0.44 to -0.46 +/- 0.23 nN in 0.01 M PBS (statistically different, Mann-Whitney rank sum test, P < 0.01). The distributions of adhesion affinities between E. coli LPS macromolecules and the AFM tip could be described by gamma distribution functions. Direct measurements of the adhesive force between E. coil and a surface were compared with adhesion in batch and column experiments, and agreement was observed between the influences of LPS on adhesion in each system. Bacterial batch retention to glass or in packed beds to quartz sand decreased after LPS removal. When interaction forces were measured during the approach of the AFM tip to a bacterium, steric repulsive forces were seen fo...Continue Reading

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Citations

Apr 23, 2013·Biosensors & Bioelectronics·Ming Soon ChengChee-Seng Toh
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