PMID: 2106918Mar 12, 1990Paper

Role of lipoxygenase in the mechanism of acrosome reaction in mammalian spermatozoa

Biochimica Et Biophysica Acta
Y LaxH Breitbart

Abstract

The acrosome reaction (AR) in bull spermatozoa was induced by the Ca2(+)-ionophore A23187, by dilauroylphosphatidylcholine or by arachidonic acid in the presence of Ca2+ in the incubation medium. The occurrence of AR was determined by following the release of acrosin from the cells. Nordihydroguaiaretic acid (NDGA), an inhibitor of both lipoxygenase and prostaglandin-synthetase, caused 35%, 43% and 69% inhibition of AR at concentrations of 1, 10 or 100 microM, respectively. Eicosatetraynoic acid (ETYA), an analogue of arachidonic acid, caused 17%, 61% and 77% inhibition of AR at concentrations of 20, 40 or 80 micrograms/ml, respectively. When AR was induced by arachidonic acid, ETYA, causes 36% and 58% inhibition at concentrations of 2 or 20 micrograms/ml, respectively. Under identical conditions, 100 microM indomethacin, a specific inhibitor of prostaglandin-synthetase, showed no inhibition but rather 35% stimulation at acrosin release rate. The fact that AR is inhibited by NDGA and not by indomethacin indicates that the lipoxygenase, rather than prostaglandin-synthetase, is involved in the mechanism of AR. Since the inhibition by NDGA is seen in the presence of the Ca-ionophore, we suggest that lipoxygenase activity is not in...Continue Reading

References

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Citations

Aug 7, 1999·Clinical Reviews in Allergy & Immunology·D J Conrad
Nov 20, 2012·Reproductive Biology and Endocrinology : RB&E·Anil K RenganStefan S du Plessis
Dec 2, 2015·Fertility and Sterility·Paula IntasquiRicardo Pimenta Bertolla
Dec 3, 2014·Biochimica Et Biophysica Acta·Marcus ConradJose Pedro Friedmann Angeli
Apr 21, 2016·Reproduction in Domestic Animals = Zuchthygiene·P CastellanosR Mateo
Sep 11, 1990·European Journal of Biochemistry·H BreitbartH A Lardy
Nov 1, 1992·The Journal of Experimental Zoology·P F WatsonP S Jones
Jul 29, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·K A FischerP Sutovsky
Sep 23, 2003·The Journal of Urology·Jennifer Tash AngerMarc Goldstein
Dec 25, 2015·Reproduction in Domestic Animals = Zuchthygiene·M C Gramajo-BühlerG Sánchez-Toranzo
Dec 23, 2020·International Journal of Molecular Sciences·Małgorzata SzczukoMaciej Ziętek
Apr 28, 1995·Biochimica Et Biophysica Acta·S RubinsteinH Breitbart

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