PMID: 994181Nov 29, 1976

Role of membrane-bound Ca in ghost permeability to Na and K

The Journal of Membrane Biology
P J Romero

Abstract

The permeability of red cell ghosts to K is determined by the amount of membrane-bound Mg which, in turn, depends on internal Mg. Contrasting with such effect, an increase in cellular Ca raises K permeability. To test whether this action is due to a competitive displacement of membrane Mg, the free Ca content of human red cell ghosts was altered by means of Ca-EGTA buffers. Net Na and K movements as well as Ca and Mg bindings were assessed after incubation in a Na-medium at 37 degrees C. Raising Ca from 3 X 10(-7) to 1 X 10(-2) M caused a large K efflux with very little Na gain. Under similar conditions, Ca binding was increased without affecting membrane-bound Mg. Both Ca binding and K loss were markedly diminished by either adding ATP to the hemolytic medium or increasing internal Mg at a fixed Ca concentration. A Scatchard analysis showed three Ca binding sites, two of them having high affinity. It is concluded that Ca action does not arise from a displacement of membrane-bound Mg but from binding to different sites in the membrane. Presumably, high affinity sites are involved in the control of K permeability.

References

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Citations

Apr 1, 1983·Pflügers Archiv : European journal of physiology·S N Orlov Postnov YuV
Nov 23, 1977·Pflügers Archiv : European journal of physiology·Y V PostnovA M Adler
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Jan 1, 1987·The Journal of Membrane Biology·B Engelmann, J Duhm
Jun 1, 1990·The Journal of Membrane Biology·P J RomeroC Melitto
Mar 14, 2013·Applied Biochemistry and Biotechnology·Pawan Kumar Maurya, Siya Prakash
Aug 10, 1990·Biochimica Et Biophysica Acta·G Benaim, P J Romero
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Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Calcium
Cell Membrane Permeability
Red Cell Ghost
Erythrocytes
Magnesium
Potassium
Sodium

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