PMID: 7541469Apr 15, 1995Paper

Role of membrane potential in endothelium-dependent relaxation of guinea-pig coronary arterial smooth muscle

The Journal of Physiology
H C ParkingtonM Tare

Abstract

1. Membrane potential and tension were measured simultaneously in ring segments of main coronary artery of guinea-pigs. The synthetic thromboxane A2 analogue U46619 depolarized the tissues from -58 +/- 2 to -40 +/- 1 mV and increased tension by 12 +/- 1 mN mm-1. Nitric oxide (NO) and Iloprost, the stable analogue of prostacyclin, evoked hyperpolarization and relaxation. 2. The concentration of NO required to evoke half-maximal hyperpolarization (EC50 of 2 x 10(-5) M) was 40-fold higher than that which was required to induce relaxation (EC50 of 5 x 10(-7) M). The EC50 for Iloprost-induced hyperpolarization (3 x 10(-8) M) was similar to that for relaxation (4 x 10(-8) M). 3. Glibenclamide (10(-6) M) abolished the hyperpolarization in response to both NO and Iloprost but was without effect on the amplitudes of the relaxations over the complete concentration-response curves. 4. Acetylcholine evoked concentration-dependent hyperpolarization and relaxation in the presence of N omega-nitro-L-arginine methyl ester (NAME; 10(-5) M) and indomethacin (10(-6) M), and these responses were attributed to endothelium-derived hyperpolarizing factor (EDHF). The hyperpolarization produced by EDHF always preceded relaxation, and relaxation never o...Continue Reading

Citations

Dec 1, 1996·Clinical and Experimental Pharmacology & Physiology·M Félétou, P M Vanhoutte
Jun 13, 2002·Clinical and Experimental Pharmacology & Physiology·Harold A ColemanHelena C Parkington
Mar 2, 1999·British Journal of Pharmacology·T ChataigneauP M Vanhoutte
Jan 19, 2005·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Yoshio TanakaLigia Toro
May 10, 2017·Journal of Cardiovascular Pharmacology·Julie K Freed, David D Gutterman
Feb 1, 1997·British Journal of Pharmacology·Q Liu, N A Flavahan
Nov 18, 2000·British Journal of Pharmacology·F PourageaudJ L Freslon
Jun 29, 2004·Journal of Applied Physiology·Johnathan D TuneEric O Feigl
Apr 1, 1997·British Journal of Pharmacology·J PeterssonE D Högestätt
Dec 19, 2001·American Journal of Physiology. Heart and Circulatory Physiology·Marianne TareHelena C Parkington
Sep 14, 1999·The American Journal of Physiology·Y NishikawaW M Chilian
Oct 16, 1999·The Journal of Physiology·A K Hammarström, P W Gage
Mar 22, 2002·Clinical and Experimental Pharmacology & Physiology·Chris R Triggle, Hong Ding
Feb 24, 2004·The Journal of Pharmacology and Experimental Therapeutics·Glaucia E CalleraLusiane M Bendhack
Oct 26, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·L P ThompsonC P Weiner
Apr 13, 2010·Pflügers Archiv : European journal of physiology·Gillian EdwardsArthur H Weston
Oct 14, 2004·Clinical and Experimental Pharmacology & Physiology·Harold A ColemanHelena C Parkington
Mar 17, 1999·British Journal of Pharmacology·A K HammarströmH A Coleman
Feb 17, 1998·Biochemical Pharmacology·J QuilleyJ C McGiff
Jul 9, 1997·European Journal of Pharmacology·D K DekaS K Mishra

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.