Role of microRNA-130a in the pathogeneses of obstructive sleep apnea hypopnea syndrome-associated pulmonary hypertension by targeting the GAX gene.

Medicine
Zhe AnJin-Ling Fu

Abstract

The purpose of this study was to elucidate the role of microRNA-130a (miR-130a) in obstructive sleep apnea hypopnea syndrome (OSAHS)-associated pulmonary hypertension (PHT) by targeting the growth arrest-specific homeobox (GAX) gene. A total of 108 patients with OSAHS-associated PHT were recruited as the OSAHS-associated PHT group and 110 healthy individuals were randomly selected as the normal control group. Human umbilical vein endothelial cells (HUVECs) were selected and divided into the control, miR-130a mimic, mimic negative control (NC), miR-130a inhibitor, and inhibitor-NC groups. The dual luciferase reporter gene assay was used to identify the relationship between miR-130a and the GAX gene. The quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting were applied for the relative expressions of miR-130a and the mRNA and protein expressions of GAX. Serum levels of endothelin-1 (ET-1), vascular endothelial growth factor (VEGF), nitric oxide (NO), and super oxide dismutase (SOD) were detected. Cell apoptosis and angiogenic activity were analyzed by flow cytometry and cell tube formation assay. GAX was a target gene of miR-130a. Compared with the normal control group, the relative expression of miR-13...Continue Reading

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Citations

Jun 9, 2018·Circulation Research·John S Floras
Aug 22, 2018·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Sartaj KhuranaSudeep Bose
Jun 19, 2019·International Journal of Molecular Sciences·Yung-Che ChenMeng-Chih Lin
Apr 6, 2021·Aging and Disease·Yayong Li, Yina Wang
Nov 9, 2021·Expert Review of Molecular Diagnostics·Sartaj KhuranaSudeep Bose

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Methods Mentioned

BETA
transfection
PCR
electrophoresis
ELISA
flow cytometry

Software Mentioned

Cell Quest
Primer
Image J

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