Role of microRNA-23b in flow-regulation of Rb phosphorylation and endothelial cell growth.

Proceedings of the National Academy of Sciences of the United States of America
Kuei-Chun WangShu Chien

Abstract

MicroRNAs (miRs) can regulate many cellular functions, but their roles in regulating responses of vascular endothelial cells (ECs) to mechanical stimuli remain unexplored. We hypothesize that the physiological responses of ECs are regulated by not only mRNA and protein signaling networks, but also expression of the corresponding miRs. EC growth arrest induced by pulsatile shear (PS) flow is an important feature for flow regulation of ECs. miR profiling showed that 21 miRs are differentially expressed (8 up- and 13 downregulated) in response to 24-h PS as compared to static condition (ST). The mRNA expression profile indicates EC growth arrest under 24-h PS. Analysis of differentially expressed miRs yielded 68 predicted mRNA targets that overlapped with results of microarray mRNA profiling. Functional analysis of miR profile indicates that the cell cycle network is highly regulated. The upregulation of miR-23b and miR-27b was found to correlate with the PS-induced EC growth arrest. Inhibition of miR-23b using antagomir-23b oligonucleotide (AM23b) reversed the PS-induced E2F1 reduction and retinoblastoma (Rb) hypophosphorylation and attenuated the PS-induced G1/G0 arrest. Antagomir AM27b regulated E2F1 expression, but did not aff...Continue Reading

References

Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·K LinS Chien
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·G Garcia-CardeñaM A Gimbrone
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·S M McCormickK K Chittur
Sep 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tzung K HsiaiChih-Ming Ho
Dec 10, 2003·Genome Génome / Conseil National De Recherches Canada·G Brian Golding
Dec 31, 2003·Cell·Benjamin P LewisChristopher B Burge
Apr 5, 2005·Nature Genetics·Azra KrekNikolaus Rajewsky
Jun 10, 2005·Nature·Jun LuTodd R Golub
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Dec 31, 2005·Nucleic Acids Research·Minoru KanehisaMika Hirakawa
Feb 10, 2006·Biochemical and Biophysical Research Communications·Nanping WangShu Chien
Apr 15, 2006·Circulation Research·Shu Chien
Jul 20, 2006·Blood·Laura PolisenoGiuseppe Rainaldi
Aug 29, 2006·Oncogene·C Giacinti, A Giordano
Nov 14, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Shu Chien
Mar 24, 2007·Circulation Research·Yajaira SuárezWilliam C Sessa
May 1, 2007·Nature Medicine·Alessandra CarèGianluigi Condorelli
Dec 11, 2007·Trends in Pharmacological Sciences·Angelika KuehbacherStefanie Dimmeler
Feb 9, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Shani ShiloChandan K Sen
Jun 14, 2008·Cardiovascular Research·Carmen UrbichStefanie Dimmeler
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Feb 28, 2009·Circulation Research·Yajaira Suárez, William C Sessa
Jun 25, 2009·Journal of Biosciences·M Poznic
Aug 20, 2009·The Journal of Clinical Investigation·Michael S Parmacek
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xiaomei QinNanping Wang

❮ Previous
Next ❯

Citations

Jun 30, 2012·Biomechanics and Modeling in Mechanobiology·Beth L Roman, Kerem Pekkan
Oct 26, 2013·Current Atherosclerosis Reports·Xinghui SunMark W Feinberg
Feb 14, 2012·Nature Cell Biology·Eduard HergenreiderStefanie Dimmeler
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xiaomei QinNanping Wang
Jun 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jing ZhouShu Chien
Sep 10, 2010·The Journal of Biological Chemistry·Yanfei XuAlex C Minella
Apr 25, 2013·Cardiovascular Research·Peter NethChristian Weber
Aug 23, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ying-Jie GuanQian Chen
May 18, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yan HuangSamir N Ghadiali
Feb 23, 2013·Journal of Cardiovascular Pharmacology·Hui-Bin LiuZhi-Ren Zhang
Jun 28, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Casey J HollidayRobert M Nerem
Mar 8, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Chih-Wen NiHanjoong Jo
Jul 22, 2011·American Journal of Physiology. Renal Physiology·Abolfazl ZarjouGang Liu
Aug 31, 2012·Journal of Biomedical Science·Li-Jing ChenJeng-Jiann Chiu
Oct 11, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Vignesh ViswanathanBruce M Boman
Sep 11, 2014·International Journal of Molecular Sciences·Darukeshwara JoladarashiPrasanna Krishnamurthy
Jan 31, 2016·Biochimica Et Biophysica Acta·Elisa Araldi, Yajaira Suárez
May 23, 2013·Journal of Cellular Physiology·Jiao LiShi-Ming Liu
Sep 20, 2012·Biochemical and Biophysical Research Communications·Kui ChenChengheng Hu
Aug 2, 2011·Vascular Pharmacology·Dorothee Hartmann, Thomas Thum
Oct 26, 2014·Genome Biology·Mark MenorLana X Garmire
Jan 24, 2015·Hämostaseologie·L Natarelli, A Schober
Sep 8, 2012·Thrombosis and Haemostasis·Reinier A BoonStefanie Dimmeler
Aug 24, 2010·BioFactors·Christoph A SchmittVerena M Dirsch
Apr 5, 2013·Journal of Cellular and Molecular Medicine·Li-Jing ChenJeng-Jiann Chiu
Dec 6, 2011·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Claudia BangThomas Thum
Feb 26, 2016·Cardiovascular Research·S M J WeltenA Y Nossent
Sep 10, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Bin ZuoXiao Dong Chen
Dec 2, 2015·Infectious Agents and Cancer·Gabriela Elizabeth Campos-ViguriGloria Fernández-Tilapa
Nov 12, 2015·BioMed Research International·Philipp PfeiferFelix Jansen
Jun 4, 2013·Free Radical Biology & Medicine·Traci MarinJohn Y-J Shyy
Apr 23, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Aránzazu Chamorro-JorganesYajaira Suárez
Dec 4, 2014·Expert Opinion on Therapeutic Targets·Xavier LoyerAlain Tedgui
Apr 10, 2015·Nature Reviews. Cardiology·Andreas SchoberChristian Weber
Feb 16, 2015·Cellular and Molecular Life Sciences : CMLS·Jing WangQingfeng Li

❮ Previous
Next ❯

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