Role of MIF/CD74 signaling pathway in the development of pleural mesothelioma

Oncotarget
Cintia D'Amato-BritoV Serre-Beinier

Abstract

Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine implicated in acute and chronic inflammatory diseases. MIF is overexpressed in various tumors. It displays a number of functions that provide a direct link between the process of inflammation and tumor growth. Our group recently identified the MIF-receptor CD74 as an independent prognostic factor for overall survival in patients with malignant pleural mesothelioma.In the present study, we compared the levels of expression of MIF and CD74 in different human mesothelioma cell lines and investigated their physiopathological functions in vitro and in vivo.Human mesothelioma cells expressed more CD74 and secreted less MIF than non tumoral MeT5A cells, suggesting a higher sensitivity to MIF. In mesothelioma cells, high MIF levels were associated with a high multiplication rate of cells. In vitro, reduction of MIF or CD74 levels in both mesothelioma cell lines showed that the MIF/CD74 signaling pathway promoted tumor cell proliferation and protected MPM cells from apoptosis. Finally, mesothelioma cell lines expressing high CD74 levels had a low tumorigenic potential after xenogeneic implantation in athymic nude mice.All these data highlight the complexity of t...Continue Reading

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Citations

Jun 29, 2018·International Journal of Molecular Sciences·Didier J ColinVéronique Serre-Beinier
Mar 27, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Laura Florez-SampedroBarbro N Melgert

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Methods Mentioned

BETA
transfection
flow cytometry
ELISA
xenografts
xenograft
xenografting
biopsies
Protein Assay
fluorescence microscopy

Software Mentioned

Primer Express
Quantity One
LabPET
GeNorm
BD Accuri [UNK] C6
Triumph XO
Osirix
Vivid

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