Role of mitogen-activated protein kinases in the mechanism of oxidant-induced cell swelling in cultured astrocytes

Journal of Neuroscience Research
M MoriyamaMichael D Norenberg

Abstract

Cytotoxic brain edema, usually a consequence of astrocyte swelling, is an important complication of stroke, traumatic brain injury, hepatic encephalopathy, and other neurological disorders. Although mechanisms underlying astrocyte swelling are not fully understood, oxidative stress (OS) has generally been considered an important factor in its pathogenesis. To better understand the mechanism(s) by which OS causes cell swelling, we examined the potential involvement of mitogen-activated protein kinases (MAPKs) in this process. Cultures exposed to theoxidant H(2)O(2) (10, 25, 50 microM) for different time periods (1-24 hr) significantly increased cell swelling in a triphasic manner. Swelling was initially observed at 10 min (peaking at 30 min), which was followed by cell shrinkage at 1 hr. A subsequent increase in cell volume occurred at approximately 6 hr, and the rise lasted for at least 24 hr. Cultures exposed to H(2)O(2) caused the activation of MAPKs (ERK1/2, JNK and p38-MAPK), whereas inhibition of MAPKs diminished cell swelling induced by 10 and 25 microM H(2)O(2). These findings suggest that activation of MAPKs is an important factor in the mediation of astrocyte swelling following oxidative stress.

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Citations

Oct 31, 2012·Journal of Inherited Metabolic Disease·Olivier BraissantCristina Cudalbu
Nov 13, 2012·Experimental Cell Research·Samanta Oliveira LoureiroAngela T S Wyse
Jul 19, 2015·Asian Journal of Surgery·Evren DilektasliCandan Ozogul
Apr 14, 2016·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Congyu JiangShenyi Kuang
Feb 12, 2013·Brain Research·Brian T HawkinsGregory J Del Zoppo
May 29, 2021·Biological Chemistry·Dieter HäussingerBoris Görg

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