Role of MMP-9 in epithelial-mesenchymal transition of thyroid cancer.

World Journal of Surgical Oncology
Yuanchun LiTiejun Li

Abstract

The purpose of this study is to explore the role and mechanism of MMP-9 in the EMT process of thyroid cancer (TC), so as to provide a basis for clinical exploration of invasion and metastasis process of TC, looking for biological markers of tumor metastasis and molecular intervention therapy. Western blot and RT-PCR were employed to detect the expression of MMP-9 in human normal thyroid cell line HT-ori3 and human TC cell lines IHH-4 (PTC), FTC-133, and 8505C. Expression levels of EMT-related markers: epithelial cell marker E-cadherin and stromal cell marker Vimentin in TGF-1-induced TC cell lines were detected by Western blot and RT-PCR, respectively. The effects of MMP-9 downregulation on cell invasion and metastasis were investigated by wound-healing assay and cell invasion experiment. The protein and mRNA expression levels of MMP-9 in TC cell lines were increased compared with the human normal thyroid cell line HT-ori3. When TGF-β1 was added, the expression of EMT and Vimentin increased while the expression of E-cadherin decreased. Compared with the control group, the TC cells stably transfected with MMP-9 shRNA showed inhibited EMT, decreased Vimentin expression, and increased E-cadherin expression. The induction of TGF-β1...Continue Reading

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Citations

Apr 4, 2021·Cancers·Zoi PiperigkouNikos K Karamanos
Jun 3, 2021·Journal of Clinical Medicine·Katarzyna Wieczorek-Szukala, Andrzej Lewinski
Jul 11, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Sepideh MirzaeiGautam Sethi

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

ImageJ
GraphPad Prims

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