Role of monovalent and divalent metal cations in human ribokinase catalysis and regulation

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Diego Quiroga-RogerVictoria Guixé

Abstract

Human ribokinase (RK) is a member of the ribokinase family, and is the first enzyme responsible for D-ribose metabolism, since D-ribose must first be converted into D-ribose-5-phosphate to be further metabolized and incorporated into ATP or other high energy phosphorylated compounds. Despite its biological importance, RK is poorly characterized in eukaryotes and especially in human. We have conducted a comprehensive study involving catalytic and regulatory features of the human enzyme, focusing on divalent and monovalent metal regulatory effects. Mg(2+), Mn(2+), and Co(2+) support enzyme activity although at different rates, with Mn(2+) being the most effective. Analysis of the divalent cation requirement in the wild type enzyme demonstrates that in addition to that chelated by the nucleotide substrate, an activating cation (either Mn(2+) or Mg(2+)) is required to obtain full activity of the enzyme, with the affinity for both divalent cations being almost the same (4 and 8 µM respectively). Besides metal cation activation, inhibition of the enzyme activity by increasing concentrations of Mn(2+) but not Mg(2+) is observed. Also the role of residues N199 and E202 of the highly conserved NXXE motif present at the active site has b...Continue Reading

References

Jan 1, 1980·Annual Review of Biochemistry·J R Knowles
Feb 12, 1998·Protein Science : a Publication of the Protein Society·J A SigrellS L Mowbray
Sep 21, 2001·Journal of Protein Chemistry·M C Maj, R S Gupta
Jan 12, 2002·Journal of Molecular Biology·C Evalena Andersson, Sherry L Mowbray
Sep 11, 2002·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Michael E Maguire, James A Cowan
Mar 8, 2006·The FEBS Journal·Maria G TozziPiero L Ipata
Jun 21, 2006·Bioorganic & Medicinal Chemistry·Dmitry V ChuvikovskyIgor A Mikhailopulo
Nov 28, 2006·Neurochemistry International·Francesco BalestriMarcella Camici
Apr 27, 2007·The Journal of Biological Chemistry·Linda MiallauGordon A Leonard
Jun 15, 2007·Acta Biochimica Et Biophysica Sinica·Patrick O J OgbunudeMichael P Barrett
Aug 5, 2008·International Journal of Cardiology·Dean MacCarterJ A St Cyr
Oct 28, 2008·Nature Structural & Molecular Biology·Wei Yang
Oct 5, 2010·Archives of Biochemistry and Biophysics·Jaime Andrés Rivas-PardoVictoria Guixé
Oct 25, 2014·Environmental Health : a Global Access Science Source·Youssef OulhoteMaryse F Bouchard

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Citations

Feb 6, 2018·Acta Crystallographica. Section F, Structural Biology Communications·Santhosh GatreddiInsaf Ahmed Qureshi
Nov 8, 2019·Biochemical Society Transactions·Antonella Del-CorsoPiero Luigi Ipata
Nov 3, 2020·Beilstein Journal of Organic Chemistry·Julia N ArtsemyevaIgor A Mikhailopulo

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