Role of Myeloid Lineage Cell Autophagy in Ischemic Brain Injury

Stroke; a Journal of Cerebral Circulation
Masakazu KotodaTomoki Hashimoto

Abstract

Inflammatory cells play a significant role in secondary injury after ischemic stroke. Recent studies have suggested that a lack of autophagy in myeloid cells causes augmented proinflammatory cytokine release and prolonged inflammation after tissue injury. In this study, we investigated the roles of myeloid cell autophagy in ischemic brain injury. Focal cerebral ischemia was induced via transient middle cerebral artery occlusion in mice with autophagy-deficient myeloid lineage cells (Atg5flox/flox LysMCre+) and in their littermate controls (Atg5flox/flox). Infarct volume, neurological function, inflammatory cell infiltration, and proinflammatory cytokine expression levels were evaluated. Mice lacking autophagy in myeloid lineage cells had a lower survival rate for 14 days than control mice (20% versus 70%; P<0.05). Although there was no difference in infarct volume at 12 hours between the 2 groups, mice lacking autophagy in myeloid lineage cells had larger infarct volumes at later time points (3 and 7 days after reperfusion) with worse neurological deficit scores and lower grip test scores. There were a higher number of ionized calcium binding adaptor molecule 1-positive cells and cells expressing M1 marker CD16/32 in mice lacki...Continue Reading

References

Jan 6, 2000·Transgenic Research·B E ClausenI Förster
Aug 8, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·G Y YangY Sun
Feb 11, 2003·Cell Structure and Function·Noboru MizushimaTamotsu Yoshimori
Jan 4, 2006·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·S Thomas Carmichael
Jan 13, 2006·Nature Chemical Biology·Alexei DegterevJunying Yuan
Sep 6, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yichen LaiRobert S B Clark
Sep 2, 2008·Neurobiology of Disease·Silvia CarloniWalter Balduini
Apr 23, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·G Alexander WestMary P Stenzel-Poore
Jun 25, 2009·Annals of Neurology·Julien PuyalPeter G H Clarke
Aug 29, 2009·Autophagy·Julien Puyal, Peter G H Clarke
Oct 29, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anna SmirkinJunya Tanaka
Oct 14, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jun LiLouise D McCullough
Feb 19, 2011·Journal of Biomedicine & Biotechnology·Fudong Liu, Louise D McCullough
Nov 15, 2011·Cell·Noboru Mizushima, Masaaki Komatsu
Nov 17, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Marietta ZilleAndreas Wunder
Nov 19, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Dorien M SchrijversWim Martinet
Mar 24, 2012·Cell Metabolism·Babak RazaniClay F Semenkovich
Mar 27, 2012·Cell Metabolism·Xianghai LiaoIra Tabas
Jun 23, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vojo Deretic
Sep 1, 2012·Circulation Research·Göran K Hansson
Sep 14, 2013·Stroke; a Journal of Cerebral Circulation·Peter KraftChristoph Kleinschnitz
May 27, 2014·Journal of Immunological Methods·Clare L AbramClifford A Lowell
Jun 19, 2014·Stroke; a Journal of Cerebral Circulation·Seung-Hoon BaekOk-Nam Bae
Feb 7, 2015·Stroke; a Journal of Cerebral Circulation·Xenios MilidonisEmily S Sena
May 2, 2015·Stroke; a Journal of Cerebral Circulation·Kenji ShimadaTomoki Hashimoto
Aug 14, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiurong ZhaoJaroslaw Aronowski
Aug 27, 2015·Stroke; a Journal of Cerebral Circulation·Sheetal BodhankarHalina Offner
Sep 5, 2015·Stroke; a Journal of Cerebral Circulation·Michael GliemSebastian Jander
Dec 15, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Patrick C NahirneyCraig E Brown

❮ Previous
Next ❯

Citations

May 30, 2020·International Journal of Molecular Sciences·Claudia Espinosa-GarciaDonald G Stein
Oct 5, 2020·Trends in Neurosciences·Christian W KellerJan D Lünemann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Acute Stroke

A stroke occurs when blood supply to the brain is interrupted depriving the brain of oxygen and nutrients. This feed focuses cerebrovascular accidents including ischemic and paralytic stroke.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.