Role of N-glycosylation sites and CXC motifs in trafficking of medicago truncatula Nod factor perception protein to plasma membrane.

The Journal of Biological Chemistry
Benoit LefebvreJulie Cullimore

Abstract

The lysin motif receptor-like kinase, NFP (Nod factor perception), is a key protein in the legume Medicago truncatula for the perception of lipochitooligosaccharidic Nod factors, which are secreted bacterial signals essential for establishing the nitrogen-fixing legume-rhizobia symbiosis. Predicted structural and genetic analyses strongly suggest that NFP is at least part of a Nod factor receptor, but few data are available about this protein. Characterization of a variant encoded by the mutant allele nfp-2 revealed the sensitivity of this protein to the endoplasmic reticulum quality control mechanisms, affecting its trafficking to the plasma membrane. Further analysis revealed that the extensive N-glycosylation of the protein is not essential for biological activity. In the NFP extracellular region, two CXC motifs and two other Cys residues were found to be involved in disulfide bridges, and these are necessary for correct folding and localization of the protein. Analysis of the intracellular region revealed its importance for biological activity but suggests that it does not rely on kinase activity. This work shows that NFP trafficking to the plasma membrane is highly sensitive to regulation in the endoplasmic reticulum and h...Continue Reading

References

Jan 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S VithaD Volkmann
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J HaseloffS Hodge
Oct 13, 2000·The Plant Journal : for Cell and Molecular Biology·M IvanchenkoJ E Fowler
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·S H Shiu, A B Bleecker
May 7, 2003·Biochemistry·Kenneth J Woycechowsky, Ronald T Raines
Jun 12, 2004·Plant Physiology and Biochemistry : PPB·Matilde José-EstanyolPere Puigdomènech
Feb 22, 2005·The Plant Cell·Renier A L van der HoornJonathan D G Jones
Jul 30, 2005·Journal of Molecular Biology·Natarajan Kannan, Andrew F Neuwald
Aug 2, 2006·Trends in Cell Biology·Jérôme BoudeauDario R Alessi
Jun 26, 2007·Plant Physiology·Patrick SmitTon Bisseling
Oct 24, 2007·Journal of Experimental Botany·Enric Castells, Josep M Casacuberta
Dec 18, 2007·The Journal of Biological Chemistry·Takayuki OhnumaEtsuko Katoh
Apr 24, 2008·Molecular Microbiology·Girbe BuistOscar P Kuipers
May 1, 2008·Annual Review of Plant Biology·Giles E D Oldroyd, J Allan Downie
Jan 24, 2009·Trends in Plant Science·Richard J Pattison, Anna Amtmann
Sep 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jing LiJonathan D G Jones
Sep 19, 2009·The EMBO Journal·Vladimir NekrasovCyril Zipfel
Sep 19, 2009·The EMBO Journal·Yusuke SaijoPaul Schulze-Lefert
Dec 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xunli LuYusuke Saijo
Dec 17, 2009·The Journal of Biological Chemistry·Heidrun HäwekerAntje von Schaewen
Mar 18, 2010·Plant Biotechnology Journal·Véronique GomordLoïc Faye
Apr 23, 2010·Cellular Microbiology·Yusuke Saijo
Jul 16, 2010·The Journal of Biological Chemistry·Prem Raj B JosephKrishna Rajarathnam
Aug 27, 2010·Journal of Integrative Plant Biology·Xiufen SongChun-Ming Liu
Jan 6, 2011·The Journal of Biological Chemistry·Dörte Klaus-HeisenJulie V Cullimore
Apr 8, 2011·Molecular Plant-microbe Interactions : MPMI·Clare Gough, Julie Cullimore
Apr 30, 2011·Plant Physiology and Biochemistry : PPB·Judith FliegmannJean-Jacques Bono

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Citations

Jul 16, 2013·Trends in Plant Science·Clare Gough, Christophe Jacquet
Aug 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Angelique BroghammerJens Stougaard
Jul 1, 2014·Nature Communications·Stéphane MesnageMichael P Williamson
Jun 7, 2015·Annual Review of Phytopathology·Erik LimpensRene Geurts
Mar 25, 2015·Journal of Plant Research·Fernando IbáñezAdriana Fabra
Jan 16, 2015·Current Genetics·Gunseli Bayram AkcapinarVerena Seidl-Seiboth
Jan 8, 2015·Frontiers in Plant Science· Nathalie Leborgne-Castel, Karim Bouhidel
Jun 23, 2016·The New Phytologist·Hanna RovenichBart P H J Thomma
Aug 27, 2014·Chembiochem : a European Journal of Chemical Biology·Kasper K SørensenKnud J Jensen
Jul 20, 2018·Frontiers in Plant Science·Clare GoughJean-Jacques Bono

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