Role of Na(+)-H(+)-antiport in restitution of isolated guinea pig gastric epithelium after superficial injury

Digestive Diseases and Sciences
T JoutsiE Kivilaakso

Abstract

In addition to its pHi regulatory function Na(+)-H(+)-antiport is also involved in volume regulation of epithelial cells, particularly in neutral conditions. It is also known that the antiport is activated after ligand binding following growth factor receptor activation. The aim of the present study was to evaluate the role of the antiport in restitution of gastric mucosa and whether its activity is dependent on the type of superficial injury. Therefore the fundic epithelium of guinea pig stomach was perfused in an Ussing chamber in neutral conditions. Na(+)-H(+)- and Cl(-)-HCO3(-)-antiports were inhibited with 1.0 mM amiloride, 1.0 mM SITS, or with HCO3- removal and Na(+)-K(+)-2Cl(2-)-cotransporter with 0.3 M furosemide during 4 hr of restitution after superficial injury induced either by 1.25 M NaCl or by 1.0% Triton. Luminal exposure of the epithelium to amiloride had no effect on restitution but serosal application abolished the process completely. The inhibitory effect of amiloride was similar after both NaCl and Triton injury. The inhibition of Cl(-)-HCO3(-)-antiport with SITS interfered with the process as well, while HCO3- removal had no significant inhibitory effect, nor did the inhibition of Na(+)-K(+)-2Cl(-)-cotransp...Continue Reading

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Citations

Sep 9, 2011·The Journal of Biological Chemistry·Lin XueMarshall H Montrose
Apr 24, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Elise S DemitrackMarshall H Montrose
Dec 27, 2016·Journal of Cellular Physiology·Anja Paehler Vor der NolteUrsula Seidler
Jul 6, 2004·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Arun BhowmikHannu Paimela
Jul 5, 2007·The Journal of Pharmacology and Experimental Therapeutics·Regina RagasaSusan J Hagen
Apr 19, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Akinori YanakaNaomi Tanaka
Nov 8, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Susan J HagenDavid X Yang
Jul 12, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·H RossmannU Seidler

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