Role of NADH oxidase in the oxidative inactivation of Streptococcus salivarius fructosyltransferase.

Infection and Immunity
K AbbeC L Wittenberger

Abstract

A cell-associated fructosyltransferase produced by Streptococcus salivarius was irreversibly inactivated in a time-dependent manner when resting or permeabilized cell suspensions were incubated with low concentrations (less than 1.0 microM) of copper. In addition to copper, the inactivation was dependent on oxygen and on a fermentable carbon source (endogenous intracellular polysaccharide or glucose). In starved, permeabilized cell suspensions, the fermentable carbon source could be replaced by NADH but not by NADPH or ATP. Of several other S. salivarius enzymes tested, only fructosyltransferase was inactivated under these conditions. The available evidence indicated that NADH oxidase is the enzyme responsible for fructosyltransferase inactivation. Results from oxygen radical scavenger studies implicated one or more species of oxygen radicals and hydrogen peroxide in the inactivation reaction.

References

Jan 1, 1975·Methods in Enzymology·H G Lebherz, W J Rutter
May 1, 1979·Journal of General Microbiology·R R Russell
Jan 1, 1978·Journal of Bacteriology·C L WittenbergerL N Lee
Apr 1, 1972·Canadian Journal of Biochemistry·R L KhandelwalI R Hamilton
Feb 1, 1973·Archives of Oral Biology·S M Garszczynski, J R Edwards
Jul 1, 1973·Archives of Oral Biology·T Yamada, J Carlsson
Nov 1, 1973·Journal of Dental Research·C F Schachtele, J A Mayo
Jan 1, 1970·Caries Research·J Carlsson
Jun 1, 1968·Archives of Oral Biology·T DaCosta, R J Gibbons
Jul 1, 1968·Archives of Oral Biology·J van Houte, H M Jansen
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L FucciE R Stadtman
Dec 1, 1981·Journal of Bacteriology·N A Jacques, C L Wittenberger

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Citations

Sep 1, 1995·Journal of Industrial Microbiology·R E Marquis
Mar 1, 1993·The New Phytologist·Nicholas A Jacques

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