PMID: 41536May 30, 1979

Role of NADPH-dependent lipid peroxidation in the oxidase inactivation in mixed hepatic microsomal function

Bollettino della Società italiana di biologia sperimentale
C Bauer, C Leporini


When mouse liver microsomes were preincubated at 37 degrees with buffer, the monooxygenase activity was nearly constant and lipid peroxidation very low over 15 min. When preincubated with NADPH, there was an increase in lipid peroxidation accompanied by loss in enzyme activity (about 40%). The addition of EDTA 5 or 40 mM depressed lipid peroxidation and protected the monooxygenase activity with a rate increasing with concentration. The addition of FeSO4 0.5 mM had only little effect one enzyme activity whereas stimulated the response of the 2-thiobarbituric acid test.

Related Concepts

Sodium Malondialdehyde
Depressed - Symptom
Monooxygenase Activity
Lipid Peroxides
Thiobarbituric acid
Microsomes, Liver
Edetic Acid, Sodium Salt

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