Role of NADPH oxidase-2 in lipopolysaccharide-induced matrix metalloproteinase expression and cell migration

Immunology and Cell Biology
So-Yeon KimSuk-Hwan Baek

Abstract

This study examined the hypothesis that the control of NADPH oxidase-2 (Nox2)-mediated reactive oxygen species (ROS) regulates the expression of matrix metalloproteinases (MMPs) and the migration of macrophages. Lipopolysaccharide (LPS) stimulation of Raw264.7 cells and mice peritoneal macrophages increased the expression of MMP-9, 10, 12 and 13 mRNA, and also increased Raw264.7 cell migration. Treatment with an antioxidant (N-acetyl cysteine) or Nox inhibitors strongly inhibited the expression of MMPs by LPS and inhibited cell migration. LPS caused ROS production in macrophages and increased the mRNA expression of Nox isoforms Nox1 and Nox2 by 20-fold and two-fold, respectively. While Nox1 small interfering RNA (siRNA) did not inhibit LPS-mediated expression of MMPs, Nox2 siRNA inhibited the expressions of MMP-9, 10 and 12. Neither Nox1 nor Nox2 siRNA influenced the LPS-mediated expression of MMP-13. In addition, NAC or apocynin attenuated LPS-induced ROS production and MMP-9 expression. MMP-9 expression and cell migration were controlled by ERK1/2-ROS signaling. Collectively, these results suggest that LPS stimulates ROS production via ERK and induce various types of MMPs expression and cell migration.

References

Mar 17, 2000·Circulation Research·K K GriendlingM Ushio-Fukai
Sep 19, 2003·Trends in Biochemical Sciences·Gary M Bokoch, Ulla G Knaus
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tsukasa KawaharaKazuhito Rokutan
Apr 17, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Keiichiro UchikuraZhaoli Sun
Oct 9, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Lynn L StollNeal L Weintraub
Nov 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chang-Hoon WooJae-Hong Kim
Jan 27, 2005·Biochemical and Biophysical Research Communications·Maren LuchtefeldBernhard Schieffer
Jun 15, 2006·Antioxidants & Redox Signaling·Alison C CaveAjay M Shah
Oct 10, 2006·Carcinogenesis·Hirotaka Osada, Takashi Takahashi
Nov 1, 2006·The Biochemical Journal·Yalin EmreAnne-Marie Cassard-Doulcier
Dec 30, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Guillermo ZalbaJavier Díez
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Wei-Jian ZhangBalz Frei
Apr 24, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nadia AmaraJorge Boczkowski
May 29, 2007·Archives of Biochemistry and Biophysics·Samuele De Minicis, David A Brenner
Jun 2, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Katrin SchröderRalf P Brandes
Apr 26, 2008·Antioxidants & Redox Signaling·Giovanni Benard, Rodrigue Rossignol
May 20, 2008·Biochemical and Biophysical Research Communications·Kenya KimuraKen-ichi Isobe
Jul 31, 2008·Antioxidants & Redox Signaling·Laura S Haneline
Sep 5, 2008·Current Opinion in Lipidology·Ralf P Brandes, Katrin Schröder
Sep 12, 2008·Antioxidants & Redox Signaling·Randall S FreyAsrar B Malik
Nov 1, 2008·The Journal of Microbiology·Yang-Sin LeeYun-Jung Yoo
Nov 22, 2008·Journal of the American College of Cardiology·Eberhard Schulz, Thomas Münzel
Nov 26, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Houssam RaadJamel El-Benna
Dec 3, 2008·Cardiovascular Research·Masatsugu Hori, Kazuhiko Nishida
Dec 17, 2008·Antioxidants & Redox Signaling·Fredrick D OakleyJohn F Engelhardt
Dec 17, 2008·Molecular BioSystems·Najl V ValeyevDeclan G Bates
Aug 26, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alexey KuzmenkinJuergen Hescheler

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Citations

Dec 14, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Preston GoodsonMy N Helms
May 15, 2012·Circulation Research·Bernard LassègueKathy K Griendling
Dec 29, 2011·Frontiers in Bioscience (Scholar Edition)·Angelica M Amanso, Kathy K Griendling
Sep 28, 2013·Antioxidants & Redox Signaling·Katrin Schröder
Aug 31, 2010·Seminars in Immunopathology·Grace Y LamJohn H Brumell
Jan 22, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Heather MendenVenkatesh Sampath
Jan 3, 2012·Trends in Cell Biology·Thomas Ryan HurdRuth Lehmann
Jan 24, 2014·BioMed Research International·Hsi-Lung Hsieh, Chuen-Mao Yang
Mar 12, 2015·Clinical Science·Julien CachatKarl-Heinz Krause
Sep 27, 2012·Free Radical Research·Aoife Corcoran, Thomas G Cotter
May 13, 2014·Free Radical Biology & Medicine·Jinah ChoiYiyan Wang
Jun 12, 2016·Antioxidants & Redox Signaling·John D ShortReto Asmis
Oct 9, 2016·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Begoña M EscribanoIsaac Túnez
Sep 6, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Yasuyoshi KigawaAkira Miyazaki
Apr 16, 2016·Annals of Biomedical Engineering·Luke D Amer, Stephanie J Bryant

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