Role of NADPH oxidase in tissue growth in a tissue engineering chamber in rats

Journal of Tissue Engineering and Regenerative Medicine
Hiroki HachisukaFan Jiang

Abstract

Previously we described a subcutaneous arteriovenous loop (AVL)-based tissue engineering chamber system, which contains an intrinsic circulation circuit created by joining the proximal ends of the femoral artery and vein with a venous graft. We showed that nicotinamide adenine dinucleotide phosphate (NADPH) oxidase was involved in mediating neovascularization inside the chamber. However, the role of NADPH oxidase in tissue formation in the chamber is unknown. In this study, we examined the effects of gp91ds-tat, a peptidyl inhibitor of NADPH oxidase, on the growth of engineered tissue blocks, using a rat chamber model. Chambers containing the AVL were filled with Matrigel mixed with gp91ds-tat (100 microM) or the scrambled control peptide. At 14 days, in control chambers, most of the Matrigel was replaced by granulation tissues; gp91ds-tat treatment significantly reduced the level of reactive oxygen species and retarded the tissue formation process. Although the total number of blood vessels per unit cellularized area was not different between two groups, most vessels in gp91ds-tat-treated tissues had smaller lumens as compared to control. The total area occupied by vessel lumens was much less in gp91ds-tat-treated tissues (10....Continue Reading

References

Mar 17, 2000·Circulation Research·K K GriendlingM Ushio-Fukai
Jan 19, 2002·Annals of the New York Academy of Sciences·O C CassellK R Knight
Jan 14, 2004·Circulation·Tetsuro AgoMitsuo Iida
Jan 11, 2005·Cardiovascular Research·Sara H M EllmarkGrant R Drummond
May 26, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tohru AdachiSumio Kawata
Jul 15, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Aurora MessinaWayne A Morrison
Dec 19, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zerina LokmicGeraldine M Mitchell
Jan 4, 2007·Circulation·Andrew N MorrittWayne A Morrison
Jan 24, 2007·Physiological Reviews·Karen Bedard, Karl-Heinz Krause
Jun 5, 2007·European Journal of Pharmacology·Seiko ManabeJitsuo Higaki
Aug 25, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Srinivasa Raju DatlaFan Jiang

❮ Previous
Next ❯

Citations

Feb 25, 2020·Journal of Tissue Engineering and Regenerative Medicine·Heidi DebelsWayne Morrison
Feb 24, 2016·Free Radical Research·Preethi Sheshadri, Anujith Kumar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.