Role of nicotinic receptors and acetylcholine in mucous cell metaplasia, hyperplasia, and airway mucus formation in vitro and in vivo.

The Journal of Allergy and Clinical Immunology
Sravanthi GundavarapuMohan L Sopori

Abstract

Airway mucus hypersecretion is a key pathophysiologic feature in a number of lung diseases. Cigarette smoke/nicotine and allergens are strong stimulators of airway mucus; however, the mechanism of mucus modulation is unclear. We sought to characterize the pathway by which cigarette smoke/nicotine regulates airway mucus and identify agents that decrease airway mucus. IL-13 and γ-aminobutyric acid type A receptors (GABA(A)Rs) are implicated in airway mucus. We examined the role of IL-13 and GABA(A)Rs in nicotine-induced mucus formation in normal human bronchial epithelial (NHBE) and A549 cells and secondhand cigarette smoke-induced, ovalbumin-induced, or both mucus formation in vivo. Nicotine promotes mucus formation in NHBE cells; however, the nicotine-induced mucus formation is independent of IL-13 but sensitive to the GABA(A)R antagonist picrotoxin. Airway epithelial cells express α7-, α9-, and α10-nicotinic acetylcholine receptors (nAChRs), and specific inhibition or knockdown of α7- but not α9/α10-nAChRs abrogates mucus formation in response to nicotine and IL-13. Moreover, addition of acetylcholine or inhibition of its degradation increases mucus in NHBE cells. Nicotinic but not muscarinic receptor antagonists block allerge...Continue Reading

References

Feb 1, 1994·American Journal of Respiratory Cell and Molecular Biology·T Gordon, J R Harkema
May 1, 1997·Critical Reviews in Toxicology·R T Boyd
Aug 19, 1999·The American Journal of the Medical Sciences·B A MarkewitzD K Payne
Feb 3, 2000·Journal of Clinical Pathology·H M el-ZimaityD Y Graham
Mar 31, 2000·Toxicology and Applied Pharmacology·S P SinghM L Sopori
Oct 25, 2000·Neuropharmacology·M VerbitskyA B Elgoyhen
Sep 21, 2001·The Journal of the Association of Nurses in AIDS Care : JANAC·R S Ferri
Dec 26, 2001·Molecular Pharmacology·Frédéric SgardFrançois Besnard
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 30, 2002·Nature Reviews. Immunology·Mohan Sopori
Jun 10, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hazel C AthertonHenry Danahay
Sep 11, 2003·Medical Mycology·W BuzinaH Stammberger
Dec 30, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Andrew K Jones, David B Sattelle
Feb 11, 2004·Current Allergy and Asthma Reports·Marsha Wills-Karp
Feb 8, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·G DorionY Cormier
Mar 12, 2005·The Journal of Physiology·Sergio FucileFabrizio Eusebi
Feb 1, 2006·Biochemistry·Michael EllisonBaldomero M Olivera
Dec 29, 2006·Journal of Molecular Neuroscience : MN·Katrin Susanne LipsWolfgang Kummer
Jan 29, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Andrew ChurgJoanne L Wright
Jul 21, 2009·Biochemical Society Transactions·Jonathan Turner, Carol E Jones
Aug 20, 2009·Current Medicinal Chemistry·K IzuharaH Aizawa
May 8, 2010·American Journal of Respiratory Cell and Molecular Biology·Xiao Wen FuEliot R Spindel
Aug 19, 2010·Cellular Microbiology·Allison McCormickFrank Ebel
Nov 4, 2010·Current Opinion in Oncology·Jean-Marie Tournier, Philippe Birembaut
Jan 31, 2012·The Journal of Allergy and Clinical Immunology·Alan P KnutsenAndrew J Wardlaw
May 15, 2012·The Journal of Allergy and Clinical Immunology·Sravanthi GundavarapuMohan L Sopori
Apr 14, 2015·The Journal of Clinical Investigation·Priya RajaveluJeffrey A Whitsett

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Citations

Jun 4, 2014·Drugs·Clémence MartinPierre-Régis Burgel
Aug 20, 2015·International Immunopharmacology·Nikolaus P DittrichMartin Fronius
Mar 10, 2016·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·M Emura, M Aufderheide
Jul 24, 2013·Biochemical and Biophysical Research Communications·Osamu NarumotoNaomi Yamashita
May 15, 2012·The Journal of Allergy and Clinical Immunology·Sravanthi GundavarapuMohan L Sopori
Sep 7, 2016·Scientific Reports·Mei-Lin ShenDong Chuan Wu
Oct 7, 2017·Current Allergy and Asthma Reports·Phillip W Clapp, Ilona Jaspers
Oct 3, 2018·The Journal of Clinical Investigation·Hitendra S ChandMohan Sopori
Feb 1, 2019·Journal of Cellular Physiology·Khalil HajiasgharzadehBehzad Baradaran
Jan 4, 2019·Recent Patents on Anti-cancer Drug Discovery·Alessia SantoroPatrizia Russo
Mar 21, 2020·Respiratory Research·Lawrence W RasmussenS Vamsee Raju
Nov 24, 2020·Frontiers in Physiology·Tanner J Wetzel, Todd A Wyatt
Apr 8, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yael-Natalie H EscobarIlona Jaspers

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