Abstract
Nitric oxide (NO) and endothelin-1 (ET-1) have both been implicated in the pathogenesis of pulmonary hypertension (PH). Therefore, we examined NO-related relaxation and ET-1 levels in rat hilar pulmonary arteries (PA) during the progression of monocrotaline (MCT)-induced PH. Rats were studied 1 and 2 weeks after a single subcutaneous injection of MCT (80 mg/kg). Pulmonary artery pressure (PAP), right ventricular hypertrophy (RVH), NO-related relaxation and tissue ET-1 levels in PA were evaluated and compared with control (C). One week post-MCT, endothelium (E)-dependent relaxation to 10(-5) M adenosine diphosphate (ADP), 10(-5) M A23187 and 10(-5) M acetylcholine (ACh) and tissue ET-1 levels in PA were normal. Rats in this group did not develop PH or RVH. Two weeks post-MCT, E-dependent relaxation was impaired (ADP, 7 +/- 3% VS. c, 62 +/- 5%; A23187, 2 +/- 7% vs. C, 58 +/- 2%; ACh, 33 +/- 7% vs. C, 86 +/- 2%; P < 0.05) and ET-1 levels were elevated (1925 +/- 244 pg/g wwt vs. C, 469 +/- 59 pg/g wwt, P < 0.05), In addition, significant PH and RVH were present (PAP 33 +/- 4 mmHg vs. C 18 +/- 0.8 mmHg, P < 0.05; RVH index 0.40 +/- 0.006 vs. C, 0.25 +/- 0.01, P < 0.05). Incubation with 10 microM indomethacin, 150 U/ml superoxide dis...Continue Reading
Citations
Oct 31, 2002·American Journal of Respiratory and Critical Care Medicine·Toshihiko NishimuraPeter N Kao
Nov 11, 1999·British Journal of Pharmacology·H NakazawaH Karaki
Dec 4, 2002·Environmental Health Perspectives·Joao R F BatalhaJohn J Godleski
Feb 28, 2002·British Journal of Pharmacology·Graeme A DeucharMartin N Hicks
Jul 15, 2009·Hypertension·Anna CsiszarZoltan Ungvari
Jun 11, 2011·Pulmonary Medicine·Rajamma Mathew
Oct 1, 1998·European Journal of Pharmacology·F YoshiharaK Kangawa
Sep 1, 2005·Toxicology and Applied Pharmacology·Wei ChengYushi Ito
Jun 28, 2000·Journal of the Autonomic Nervous System·P M Vanhoutte
Nov 27, 2015·Pulmonary Pharmacology & Therapeutics·Poorella LingeshwarKashif Hanif
Apr 24, 2008·Clinical and Experimental Pharmacology & Physiology·Maristela P Souza-RabboAdriane Belló-Klein
Apr 22, 2010·Italian Journal of Pediatrics·Nagla T El-Melegy, Nagwa A Mohamed
Mar 3, 2005·European Journal of Pharmacology·Charlotte U AndersenUlf Simonsen
Nov 3, 2009·Journal of Pharmacological Sciences·Fusae SawamuraAnthony Beardsworth
Feb 2, 2013·Pulmonary Circulation·Narendra DereddyRajamma Mathew
Mar 15, 2011·Cell Transplantation·Philipp JungebluthPaolo Macchiarini
Feb 18, 2004·Pediatric Pulmonology·Zen-Kong DaiJiunn-Ren Wu
Jun 17, 1998·Circulation·S PriéJ Dupuis
Jan 25, 2006·Respiration; International Review of Thoracic Diseases·Noriyuki HommaYoshinosuke Fukuchi
Apr 18, 2007·Cardiology in Review·Rajamma MathewMichael H Gewitz
Feb 27, 2019·Pulmonary Circulation·Jing HuangRajamma Mathew
Sep 19, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Syed R BaberPhilip J Kadowitz
Apr 24, 2019·Experimental Lung Research·Jana VeteskovaJan Klimas
Oct 8, 1999·Anesthesia and Analgesia·D J HorstmanG F Rich
Aug 21, 2013·Journal of Synchrotron Radiation·Emily A GrayDaryl O Schwenke
Oct 29, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Rajamma Mathew
Feb 10, 1998·Journal of Cardiovascular Pharmacology·L BrownC Sernia