Role of nitric oxide in heat shock protein induced apoptosis of gammadeltaT cells

International Journal of Cancer. Journal International Du Cancer
Nilangi AtreShubhada Chiplunkar

Abstract

Activation induced cell death (AICD) has been proposed to serve as a mechanism to limit T lymphocyte proliferation induced by antigenic stimulation. Heat shock proteins (hsp60 and hsp70) expressed on oral tumor cells serve as ligands for peripheral blood gammadeltaT lymphocytes. Tumor cell lysis by gammadeltaT lymphocytes is mediated via recognition of hsp expressed on tumor cells. In the present study, we report that upon stimulation with hsp, gammadeltaT lymphocytes isolated from oral cancer patients undergo AICD as confirmed by DNA ploidy, annexin V staining and confocal microscopy. In cocultures of gammadeltaT lymphocytes and tumor cells, addition of antihsp60 and antihsp70 MAb, but not anti-Fas MAb (ZB4), inhibited DNA fragmentation of gammadeltaT lymphocytes. Flow cytometric analysis revealed a down regulation of Fas expression on gammadeltaT lymphocytes upon incubation with hsp60 and hsp70. Increased expression of iNOS was observed in hsp-stimulated gammadeltaT lymphocytes. Addition of monomethyl L-arginine monoacetate, competitive inhibitor of NOS, inhibited nitric oxide (NO) production and apoptosis of gammadeltaT lymphocytes induced by hsp60 and hsp70. The NO-induced apoptosis of gammadeltaT lymphocytes involves activ...Continue Reading

References

Mar 10, 1995·Science·S Nagata, P Golstein
Jan 1, 1993·International Archives of Allergy and Immunology·D Kabelitz
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·S H Kaufmann
Sep 12, 1997·The Journal of Biological Chemistry·C ScioratiE Clementi
Jul 22, 1998·Cancer Immunology, Immunotherapy : CII·T L Whiteside, H Rabinowich
Feb 27, 1999·International Journal of Cancer. Journal International Du Cancer·A D LaadS V Chiplunkar
Aug 24, 1999·Immunology and Cell Biology·P Waring, A Müllbacher
Apr 17, 2001·Biochemical and Biophysical Research Communications·H T ChungY M Kim
Aug 23, 2001·International Immunopharmacology·P K KimT R Billiar
Apr 3, 2002·Seminars in Cancer Biology·Theresa L Whiteside
Nov 26, 2002·Free Radical Biology & Medicine·Guy C Brown, Vilmante Borutaite
May 20, 2003·Immunological Reviews·Andreas KruegerPeter H Krammer
Feb 5, 2005·Toxicology·Gilbert A BlaiseSimon Authier
Mar 31, 2005·Veterinary Immunology and Immunopathology·Mingqi DengMitchell S Abrahamsen

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Citations

Nov 16, 2010·Veterinary Immunology and Immunopathology·Inmaculada BarrancoLibrado Carrasco
Feb 24, 2015·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Deepika Bablani PopliVatchala Rani
Nov 5, 2016·PloS One·Laetitia DouguetArmelle Prévost-Blondel
Nov 6, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tawanda ZiningaAddmore Shonhai
Jul 20, 2010·Immunotherapy·Shubhada ChiplunkarDieter Kabelitz

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