Role of nitric oxide in lung ischemia and reperfusion injury

The American Journal of Physiology
T M MooreA E Taylor

Abstract

We studied the effects of nitric oxide synthase (NOS) inhibitors and nitric oxide (NO.) donors on ischemia-reperfusion (I/R)-induced microvascular permeability increase in isolated buffer-perfused rat lungs. Microvascular permeability (Kf,c) was significantly increased in lungs subjected to 45 min of ischemia followed by 30 min of reperfusion. Lungs that were pretreated with 300 and 600 microM N omega-nitro-L-arginine methyl ester (L-NAME), 1, 300, and 600 microM NG-monomethyl-L-arginine (L-NMMA), or 600 microM L-N6-(1-iminoethyl) ornithine (L-NIO) still showed significant increases in Kf,c after I/R. Lungs that were pretreated with 5 mM L-NAME or 5 mM N omega-nitro-D-arginine methyl ester showed no increase in Kf,c after I/R. However, both compounds at these concentrations produced significant decreases in perfusate pH. The decreased pH was responsible for the protective effects, since lungs pretreated with 5 mM L-NAME and supplemented with NaHCO3 to prevent the perfusate pH decrease still showed a significant elevation in Kf,c after I/R. In additional experiments, NO.donors were administered to isolated lungs at the onset of reperfusion. Spermine-NO (100 microM) and S-nitroso-N-acetylpenacillamine (300 microM) both prevented ...Continue Reading

Citations

Jun 12, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Aubrey E Taylor
Sep 14, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Willem A den HengstAn L Moens
Aug 25, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·H SchütteF Grimminger
Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·D B Pearse, P M Becker
Jan 13, 2004·Journal of Applied Physiology·B D NossamanP J Kadowitz
Jul 19, 2000·American Journal of Physiology. Heart and Circulatory Physiology·J M Dodd-O, D B Pearse
Feb 24, 2007·Journal of Applied Physiology·Jiunn-Song JiangChung-Ming Chen

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