Role of nitric oxide in relaxation of the longitudinal layer of rectal smooth muscle

Diseases of the Colon and Rectum
J F StebbingN J Mortensen

Abstract

This study was designed to investigate the role of nitric oxide in neurogenic relaxation of the longitudinal layer of human rectal smooth muscle. Tissue was obtained from the mid rectum of patients undergoing anterior resection for carcinoma. Adjacent strips of longitudinal muscle were dissected and mounted in organ baths for isometric tension recording. In preliminary experiments to determine the response of strips to cholinergic, adrenergic, and potential excitatory agonists, strips were superfused with standard Krebs solution (37 +/- 0.5 degrees C; pH, 7.4 +/- 0.05). Investigation of inhibitory, nonadrenergic noncholinergic responses required the addition of 3 x 10(-6) M histamine to induce reproducible and stable tension for five-minute "test" periods, during which electrical field stimulation (EFS) and additional drugs were applied. In these experiments, strips were superfused with Krebs solution that contained atropine sulfate (3 x 10(-6) M) and guanethidine (3 x 10(-6) M). The response to cholinergic and adrenergic agonists was typical of nonsphincter specialized gastrointestinal smooth muscle. EFS elicited frequency-dependent, neurogenic (tetrodotoxin-sensitive) relaxations of precontracted strips, which were reduced in...Continue Reading

References

Nov 1, 1976·Journal of Applied Physiology·P ArhanD Pellerin
Feb 1, 1992·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·M D'AmatoP Montuschi
Mar 1, 1996·Diseases of the Colon and Rectum·J F StebbingN J Mortensen

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Citations

Jun 23, 1999·Diseases of the Colon and Rectum·T A CookN J Mortensen
Jun 20, 2000·Diseases of the Colon and Rectum·T R KochG L Telford
Jun 23, 2004·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·P J Lunniss, C V Mann
Mar 18, 2004·Movement Disorders : Official Journal of the Movement Disorder Society·Giuseppe BrisindaAlberto Albanese
Aug 3, 2002·The British Journal of Surgery·G MariaG Brisinda
Jun 11, 1999·The American Journal of Gastroenterology·L SiproudhisM Gosselin

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