Abstract
The anterior pituitary gland is a site of nitric oxide (NO) production and action, suggesting a local regulatory function. We recently reported that NO inhibits in vitro prolactin release. The aim of the present study was to establish the mechanism of action of NO on prolactin release and to determine whether NO is involved in the inhibitory effect of GABA on prolactin release. Since NO exerts its action through cGMP by activating guanylate cyclase in different tissues, we examined the effect of sodium nitroprusside (NP), a NO releaser, on intrapituitary cGMP levels. Incubation of anterior pituitary glands with 0.5 mM NP 4-fold increased intrapituitary cGMP content, but decreased intrapituitary cAMP levels. In addition, we studied the effect of NP on prolactin release in the presence of LY 83583, an inhibitor of guanylate cyclase activity and 3-isobutyl-1-methylxanthine (IBMX), an inhibitor of phosphodiesterase activity. 10 microM LY 83583 and 0.5 mM IBMX blocked the inhibitory effect of NP on prolactin release. (10(-3) M) 8Br-cGMP, an analogue of cGMP, mimicked the effect of NP on prolactin release. On the other hand, NO seems to be involved in the inhibitory effect of GABA on prolactin release since hemoglobin, a scavenger of...Continue Reading
References
Jan 1, 1977·Psychoneuroendocrinology·A GuidottiA Kurosawa
Apr 1, 1978·Journal of Neurochemistry·J R WaltersL P Miller
Dec 18, 1992·Science·J S StamlerJ Loscalzo
Nov 1, 1991·Basic Research in Cardiology·S M LohmannU Walter
Jan 1, 1989·Psychoneuroendocrinology·J A ApudG Racagni
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D S Bredt, S H Snyder
May 1, 1988·Circulation Research·C H Twort, C van Breemen
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S S RashatwarT M Lincoln
Nov 1, 1972·The Biochemical Journal·L J Fowler, R A John
Nov 10, 1983·Nature·R M RapoportF Murad
Oct 1, 1995·Physiological Reviews·J A Beavo
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S CeccatelliT Hökfelt
Mar 1, 1994·The Biochemical Journal·R G Knowles, S Moncada
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·B H DuvilanskiS M McCann
Feb 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T M Lincoln, T L Cornwell
Jan 1, 1993·Life Sciences·L BrunettiM Vacca
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·A SeilicovichS M McCann
Oct 10, 1994·Brain Research·J J BonaveraS P Kalra
Mar 1, 1995·Gastroenterology·J F DufourI M Arias
May 26, 1994·Nature·S D MerineyG R Pilar
Jan 1, 1994·Life Sciences·B BrüneE G Lapetina
Mar 3, 1995·Life Sciences·L BrunettiM Vacca
Citations
May 27, 2011·Neuroscience Bulletin·Ming-Yu Jiang, De-Pei Cai
Nov 8, 2003·Brain Research. Developmental Brain Research·A MantelasF Stylianopoulou
Jan 24, 2004·Life Sciences·Anunciación LafuenteAna I Esquifino
Feb 18, 2004·Life Sciences·A S Moreno, C R Franci
Feb 13, 2001·Molecular and Cellular Endocrinology·M O VelardezB H Duvilanski
Feb 18, 1999·Molecular and Cellular Endocrinology·B H DuvilanskiD Becu-Villalobos
Nov 23, 2006·Neuroimmunomodulation·Camila ScorticatiAndrea De Laurentiis
Jun 14, 2000·General and Comparative Endocrinology·A D Uretsky, J P Chang
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·S KaranthS M McCann
Feb 4, 2010·Toxicology·Nelson StürtzAna M Evangelista de Duffard
Jan 13, 2009·Journal of Chemical Neuroanatomy·Archana GaikwadNishikant Subhedar
Apr 11, 2012·Journal of Neuroendocrinology·A N MeintsJ P Chang
Mar 27, 2007·Brain Research·Athanasios MantelasFotini Stylianopoulou
Sep 30, 2004·Free Radical Biology & Medicine·Ariel H B PoliandriBeatriz H Duvilanski
Feb 7, 2006·Free Radical Biology & Medicine·Ariel H B PoliandriBeatriz H Duvilanski
May 18, 2016·Nigerian Medical Journal : Journal of the Nigeria Medical Association·Uduak Akpan Okon, Ikponoabasi Ibanga Utuk
May 17, 2003·Pain·D Schulte, J Millar
Mar 31, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Jill M RussellP Callahan
Nov 20, 1998·Journal of Cardiovascular Pharmacology·R LiuM Kaufmann
Jan 5, 1999·The Journal of Biological Chemistry·E A RatovitskiC J Lowenstein
Oct 4, 2000·Physiological Reviews·M E FreemanG Nagy
Mar 5, 2003·Journal of Neurochemistry·Agnese SecondoLucio Annunziato