Role of nitrite in the competition between denitrification and DNRA in a chemostat enrichment culture

AMB Express
Eveline M van den BergMark Cm van Loosdrecht

Abstract

Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are two microbial processes that compete for oxidized nitrogen compounds in the environment. The objective of this work was to determine the role of nitrite versus nitrate as terminal electron acceptor on the competition between DNRA and denitrification. Initially, a mixed culture chemostat was operated under nitrate limitation and performed DNRA. Stepwise, the influent nitrate was replaced with nitrite until nitrite was the sole electron acceptor and N-source present. Despite changing the electron acceptor from nitrate to nitrite, the dominant process remained DNRA and the same dominant organism closely related to Geobacter lovleyi was identified. Contrary to previous studies conducted with a complex substrate in marine microbial communities, the conclusion of this work is that nitrate versus nitrite as electron acceptor does not generally control the competition between DNRA and denitrification. Our results show that the effect of this ratio must be interpreted in combination with other environmental factors, such as the type and complexity of the electron donor, pH, or sulfide concentrations.

References

Jan 1, 1982·Antonie van Leeuwenhoek·J M TiedjeJ A Robinson
Nov 1, 2008·Environmental Microbiology·Mike S M Jetten
Feb 6, 2009·Biomacromolecules·Katja JohnsonMark C M van Loosdrecht
Jan 12, 2011·Journal of Biotechnology·Beate KraftHalina E Tegetmeyer
Mar 15, 2015·Applied and Environmental Microbiology·Sukhwan YoonFrank E Löffler
Apr 25, 2015·The ISME Journal·Eveline M van den BergMark Cm van Loosdrecht
Dec 10, 2016·Frontiers in Microbiology·Eveline M van den BergMark C M van Loosdrecht

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Citations

Aug 24, 2018·Environmental Microbiology·Hidetoshi UrakawaBehzad Mortazavi
Feb 7, 2019·Frontiers in Microbiology·David C VuonoJoseph J Grzymski
Jun 11, 2020·The Journal of Biological Chemistry·Julius CampeciñoEric L Hegg
Mar 25, 2019·Journal of Microbiological Methods·Jordan CannonJoanne Chee-Sanford
Oct 24, 2020·Environmental Science & Technology·Mingsheng JiaEveline I P Volcke
Oct 5, 2021·The Science of the Total Environment·N ValienteJ J Gómez-Alday
Oct 27, 2021·Environmental Science and Pollution Research International·Xiaoling LiChunshuang Liu

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BETA
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electrophoresis

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Chromas Lite
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AxioVision

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