Role of nitrogen-metabolism genes expressed during pathogenicity of the alkalinizing Colletotrichum gloeosporioides and their differential expression in acidifying pathogens

Molecular Plant-microbe Interactions : MPMI
Itay MiyaraD Prusky

Abstract

Pathogens can actively alter fruit pH around the infection site, signaling modulation of pathogenicity-factor expression, as found for alkalinizing (Colletotrichum and Alternaria spp.) and acidifying (Penicillium, Botrytis, and Sclerotinia spp.) fungi. The nitrogen-metabolism genes GDH2, GS1, GLT, and MEP genes are differentially expressed during colonization by Colletotrichum gloeosporioides, and a Δgdh2 strain reduces ammonia accumulation and pathogenicity. We analyzed the contribution of transporters GLT and MEPB to C. gloeosporiodes pathogenicity. Germinating spores of Δglt strains showed reduced appressorium formation; those of ΔmepB mutants showed rapid ammonia uptake and accumulation inside the hyphae, indicating deregulated uptake. Both mutants reduced pathogenicity, indicating that these transporters function during alkalinizing species pathogenicity. We compared the expressions of these genes in C. gloeosporioides and Sclerotinia sclerotiorum, and found five to 10-fold higher expression at the transcript level in the former. Interestingly, GLT and MEPB in the alkalinizing species showed no and very low sequence identity, respectively, with their counterparts in the acidifying species. Knockout analysis of GLT and MEPB...Continue Reading

References

Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·S Nakanishi, M Masu
May 21, 1998·Molecular Microbiology·K J Linton, C F Higgins
Jan 3, 2001·Applied and Environmental Microbiology·J A Rollins, M B Dickman
Jul 18, 2001·Fungal Genetics and Biology : FG & B·A ten HaveJ A van Kan
Aug 11, 2001·Molecular Plant-microbe Interactions : MPMI·N YakobyD Prusky
Sep 12, 2001·Molecular Plant-microbe Interactions : MPMI·D PruskyW S Conway
Jun 14, 2002·Gene·Boris Magasanik, Chris A Kaiser
Aug 17, 2002·Molecular Plant-microbe Interactions : MPMI·Dani EshelDov Prusky
Sep 26, 2002·FEBS Letters·Daniel WipfWolf B Frommer
Apr 17, 2003·Applied Microbiology and Biotechnology·Levente Karaffa, Christian P Kubicek
Jun 6, 2003·Applied and Environmental Microbiology·N DroriD Prusky
Oct 8, 2003·Systematic Biology·Stéphane Guindon, Olivier Gascuel
Jan 1, 1996·Annual Review of Phytopathology·D Prusky
Sep 14, 2004·Science·Shahram KhademiRobert M Stroud
Nov 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Lei ZhengXiao-Dan Li
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Evelyn ZientzRoy Gross
Oct 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Susana L A AndradeOliver Einsle
Nov 8, 2005·Pflügers Archiv : European journal of physiology·Fritz K Winkler
Oct 17, 2006·The Journal of Biological Chemistry·Arnaud JavelleMike Merrick
Mar 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Arnaud JavelleFritz K Winkler
Jun 14, 2008·Fungal Genetics and Biology : FG & B·Hadas ShafranAmir Sherman
Jul 12, 2008·Molecular Plant-microbe Interactions : MPMI·N AlkanD Prusky
Sep 2, 2008·Genome Research·Vardges Ter-HovhannisyanMark Borodovsky
Apr 22, 2009·The Journal of Biological Chemistry·Renae M RyanJoseph A Mindell
Nov 1, 2003·Molecular Plant Pathology·Dov Prusky, Nir Yakoby

❮ Previous
Next ❯

Citations

Aug 7, 2013·Applied Microbiology and Biotechnology·Tianyuan ZhangHongye Li
Dec 20, 2012·Antioxidants & Redox Signaling·Noam AlkanDov Prusky
May 21, 2013·Annual Review of Phytopathology·Dov PruskyRobert Fluhr
Mar 1, 2013·BMC Genomics·Naomi TrushinaBenjamin A Horwitz
May 23, 2014·Annual Review of Phytopathology·Jessie FernandezRichard A Wilson
Oct 18, 2012·Plant Signaling & Behavior·Gabriel E RechMichael R Thon
Nov 10, 2017·PLoS Pathogens·Tânia R FernandesAntonio Di Pietro
Jul 19, 2018·Insects·Bob BoogaardJan W M van Lent
Mar 7, 2017·Biochemistry. Biokhimii︠a︡·T A ValuevaN N Kudryavtseva

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.