Role of NleH, a type III secreted effector from attaching and effacing pathogens, in colonization of the bovine, ovine, and murine gut.

Infection and Immunity
Cordula HemrajaniGad Frankel

Abstract

The human pathogen enterohemorrhagic Escherichia coli (EHEC) O157:H7 colonizes human and animal gut via formation of attaching and effacing lesions. EHEC strains use a type III secretion system to translocate a battery of effector proteins into the mammalian host cell, which subvert diverse signal transduction pathways implicated in actin dynamics, phagocytosis, and innate immunity. The genomes of sequenced EHEC O157:H7 strains contain two copies of the effector protein gene nleH, which share 49% sequence similarity with the gene for the Shigella effector OspG, recently implicated in inhibition of migration of the transcriptional regulator NF-kappaB to the nucleus. In this study we investigated the role of NleH during EHEC O157:H7 infection of calves and lambs. We found that while EHEC DeltanleH colonized the bovine gut more efficiently than the wild-type strain, in lambs the wild-type strain exhibited a competitive advantage over the mutant during mixed infection. Using the mouse pathogen Citrobacter rodentium, which shares many virulence factors with EHEC O157:H7, including NleH, we observed that the wild-type strain exhibited a competitive advantage over the mutant during mixed infection. We found no measurable differences i...Continue Reading

References

Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A E JerseJ B Kaper
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·K G JarvisJ B Kaper
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T K McDanielJ B Kaper
Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper
Aug 26, 1998·Infection and Immunity·E A Dean-NystromA D O'Brien
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Apr 16, 2002·Infection and Immunity·Nancy A CornickHarley W Moon
Sep 25, 2003·International Journal of Medical Microbiology : IJMM·Martin J WoodwardRoberto M La Ragione
Feb 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Wanyin DengB Brett Finlay
Jan 18, 2005·FEMS Microbiology Reviews·Huiwen Deborah Chen, Gad Frankel
Aug 23, 2005·Infection and Immunity·Francis GirardJohn M Fairbrother
Sep 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Dong Wook KimClaude Parsot
Nov 29, 2005·Cellular Microbiology·Rosanna MundySiouxsie Wiles
Aug 24, 2006·Infection and Immunity·Siouxsie WilesGad Frankel
Sep 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Toru TobeMark J Pallen
Mar 14, 2007·Applied and Environmental Microbiology·Francis GirardGad Frankel
Jan 19, 2008·Cellular Microbiology·Oliver MarchèsEmmanuelle Caron

❮ Previous
Next ❯

Citations

Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Cordula HemrajaniGad Frankel
Sep 10, 2009·Foodborne Pathogens and Disease·Rodney A MoxleyDragan Rogan
Dec 31, 2009·PLoS Pathogens·Xiaofei GaoPhilip R Hardwidge
Nov 26, 2010·Molecular Microbiology·Sandhya V RoyanGail A Hecht
Apr 15, 2011·Molecular Microbiology·Alexander R C WongElizabeth L Hartland
Oct 20, 2010·FEMS Microbiology Reviews·Nuria AndreuSiouxsie Wiles
May 5, 2012·Gut Microbes·Abigail ClementsGad Frankel
Jan 16, 2009·Current Opinion in Microbiology·Paul Dean, Brendan Kenny
Sep 9, 2016·Nature Protocols·Valerie F CrepinGad Frankel
Sep 28, 2017·Infection and Immunity·Bruna C LustriCristiano G Moreira
Jun 9, 2016·MBio·Cristiano G MoreiraVanessa Sperandio
Jul 3, 2019·Infection and Immunity·Aman KumarVanessa Sperandio
Apr 10, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·U NaseerL T Brandal
Jul 28, 2020·Future Microbiology·Anna Katharina Riebisch, Sabrina Mühlen
Jun 25, 2015·Microbiology Spectrum·Jaclyn S Pearson, Elizabeth L Hartland
Jun 25, 2015·Microbiology Spectrum·Jay L Mellies, Emily Lorenzen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.