PMID: 7932237Jun 15, 1994Paper

Role of non-quantal acetylcholine release in surplus polarization of mouse diaphragm fibres at the endplate zone

The Journal of Physiology
Eugeny E NikolskyF Vyskocil

Abstract

1. In mouse diaphragm, with intact cholinesterase (ChE), the mean value of the resting membrane potential was significantly higher (-84.8 +/- 0.3 mV; mean +/- S.E.M.) at the endplate zone than in the extrajunctional area of the muscle fibres (-82.5 +/- 0.3 mV) at 22 degrees C. 2. This hyperpolarization of about 2-3 mV at the endplate zone was abolished within 5 min by 1 x 10(-6) M ouabain, indicating that it might be caused by an electrogenic Na(+)-K+ pump. (+)-Tubocurarine (TC; 1 x 10(-5) M) had no effect on this hyperpolarization after bath application for 10-20 min. 3. Short-term denervation (4 h), a slight increase of Mg2+ in the bath of from 1 to 4 mM and application of a Ca(2+)-free solution for 60 min also led to the disappearance of the surplus polarization. All of these factors are known to eliminate TC-induced hyperpolarization in anti-ChE-treated muscles (H-effect), which is considered to be a correlate of non-quantal acetylcholine (ACh) leakage. 4. The time courses of the decline of the H-effect and surplus polarization after denervation were identical. 5. In short-term denervated muscles with intact ChE, the surplus polarization was restored by 5 x 10(-8) M ACh, which simulates the H-effect in anti-ChE-treated musc...Continue Reading

Citations

Jan 13, 2016·The Journal of General Physiology·Violetta V KravtsovaIgor I Krivoi
Apr 13, 2000·The European Journal of Neuroscience·M R MukhtarovF Vyskocil
Mar 19, 2003·Journal of Neurochemistry·Artem I MalomouzhAlbert K Urazaev
Jan 13, 2015·Bulletin of Experimental Biology and Medicine·V V KravtsovaI I Krivoi
Jun 3, 2016·Frontiers in Physiology·Vladimir V Matchkov, Igor I Krivoi
Jun 26, 2001·The European Journal of Neuroscience·A V GalkinF Vyskocil
Jul 16, 2004·American Journal of Physiology. Cell Physiology·Tatiana L RadzyukevichJudith A Heiny
Apr 26, 2006·Pflügers Archiv : European journal of physiology·Igor I KrivoiFrederic Mandel
Aug 3, 2007·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·A I MalomouzhE E Nikolsky
Jan 10, 2012·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·V V KravtsovaI I Krivoi
May 12, 2016·American Journal of Physiology. Endocrinology and Metabolism·Sergej Pirkmajer, Alexander V Chibalin
Mar 3, 2019·International Journal of Molecular Sciences·Igor I Krivoi, Alexey M Petrov
Oct 24, 1997·Journal of Applied Physiology·S J Hong, C C Chang

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