PMID: 9443938Jan 28, 1998Paper

Role of oligosaccharides in the pharmacokinetics of tissue-derived and genetically engineered cholinesterases

Molecular Pharmacology
A SaxenaB P Doctor

Abstract

To understand the role of glycosylation in the circulation of cholinesterases, we compared the mean residence time of five tissue-derived and two recombinant cholinesterases (injected intravenously in mice) with their oligosaccharide profiles. Monosaccharide composition analysis revealed differences in the total carbohydrate, galactose, and sialic acid contents. The molar ratio of sialic acid to galactose residues on tetrameric human serum butyrylcholinesterase, recombinant human butyrylcholinesterase, and recombinant mouse acetylcholinesterase was found to be approximately 1.0. For Torpedo californica acetylcholinesterase, monomeric and tetrameric fetal bovine serum acetylcholinesterase, and equine serum butyrylcholinesterase, this ratio was approximately 0.5. However, the circulatory stability of cholinesterases could not be correlated with the sialic acid-to-galactose ratio. Fractionation of the total pool of oligosaccharides obtained after neuraminidase digestion revealed one major oligosaccharide for human serum butyrylcholinesterase and three or four major oligosaccharides in other cholinesterases. The glycans of tetrameric forms of plasma cholinesterases (human serum butyrylcholinesterase, fetal bovine serum acetylcholin...Continue Reading

References

Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M TakeuchiA Kobata
Nov 1, 1987·Analytical Biochemistry·B P DoctorI Silman
Jan 1, 1982·Annual Review of Biochemistry·G Ashwell, J Harford
Dec 1, 1993·Pharmacology, Biochemistry, and Behavior·R BrandeisY Ashani
Sep 1, 1993·Journal of Neurochemistry·J LiaoU Brodbeck
Apr 1, 1996·Journal of Pharmaceutical Sciences·P B Laub, J M Gallo
Mar 14, 1997·The Journal of Biological Chemistry·P M RuddR A Dwek
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE

❮ Previous
Next ❯

Citations

Jul 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Hong SunStephen Brimijoin
May 4, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tama EvronTsafrir S Mor
Oct 7, 2004·Molecular Pharmacology·Yang GaoStephen Brimijoin
Jun 28, 2011·Arhiv za higijenu rada i toksikologiju·Anita BosakZrinka Kovarik
Jan 19, 2008·Proteomics·Daniel KolarichFriedrich Altmann
Aug 15, 2012·The FEBS Journal·Kevser BiberogluOksana Lockridge
Apr 11, 2000·Microscopy Research and Technique·C Legay
Jul 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yue-Jin HuangSolomon Langermann
May 30, 2009·The Journal of Pharmacology and Experimental Therapeutics·Yang Gao, Stephen Brimijoin
Jan 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Denis G IlyushinAlexander G Gabibov
May 23, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Lee Koetzner, James H Woods
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Brian C GeyerTsafrir S Mor
Nov 23, 2020·Journal of Neurochemistry·Miguel Ricardo Leung, Tzviya Zeev-Ben-Mordehai
Nov 2, 2018·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Oksana LockridgeFlorian Nachon
May 1, 2009·The Journal of Physical Chemistry. B·Yongmei PanChang-Guo Zhan
Dec 25, 2008·BMC Biotechnology·Ryan R WoodsTsafrir S Mor
Jul 8, 2008·Protein Expression and Purification·Ashima SaxenaBhupendra P Doctor

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.