Role of overexpressed CFA/I fimbriae in bacterial swimming.

Physical Biology
Ling CaoXinghong Yang

Abstract

Enterotoxigenic Escherichia coli CFA/I is a protective antigen and has been overexpressed in bacterial vectors, such as Salmonella Typhimurium H683, to generate vaccines. Effects that overexpressed CFA/I may engender on the bacterial host remain largely unexplored. To investigate, we constructed a high CFA/I expression strain, H683-pC2, and compared it to a low CFA/I expression strain, H683-pC, and to a non-CFA/I expression strain, H683-pY. The results showed that H683-pC2 was less able to migrate into semisolid agar (0.35%) than either H683-pC or H683-pY. Bacteria that migrated showed motility halo sizes of H683-pC2 < H683-pC < H683-pY. In the liquid culture media, H683-pC2 cells precipitated to the bottom of the tube, while those of H683-pY did not. In situ imaging revealed that H683-pC2 bacilli tended to auto-agglutinate within the semisolid agar, while H683-pY bacilli did not. When the cfaBE fimbrial fiber encoding genes were deleted from pC2, the new plasmid, pC2(-), significantly recovered bacterial swimming capability. Our study highlights the negative impact of overexpressed CFA/I fimbriae on bacterial swimming motility.

References

Aug 1, 1992·Canadian Journal of Microbiology·J Betts, B B Finlay
Feb 1, 1990·Microbial Pathogenesis·P H SavelkoulW Gaastra
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·E T SaulinoS J Hultgren
Sep 5, 2001·The EMBO Journal·X LiH L Mobley
Nov 27, 2002·Applied and Environmental Microbiology·Gunjan Pandey, Rakesh K Jain
Jun 1, 1936·Journal of Bacteriology·R P Tittsler, L A Sandholzer
Jul 6, 2006·Applied and Environmental Microbiology·Mingming WuMatthew P DeLisa
Jan 5, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xinghong YangDavid W Pascual
Jan 24, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Zhiyong SuoAndrew Steele
Jan 30, 2007·Microbiology·Graham P Stafford, Colin Hughes
Mar 7, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Zhiyong SuoDavid W Pascual
Apr 1, 2008·Cellular Microbiology·Elisabeth Ashman Epstein, Matthew R Chapman
Jul 9, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Javier Ochoa-RepárazDavid W Pascual
Aug 18, 2009·Analytical Chemistry·Zhiyong SuoYves Idzerda
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jonathan P CelliRama Bansil
Oct 27, 2009·Journal of Bacteriology·Behnam ZolghadrSonja-Verena Albers
Oct 12, 2010·Science·Maxsim L GibianskyGerard C L Wong
Mar 23, 2011·Journal of Bacteriology·Fanhong MengCaitilyn Allen
Oct 19, 2011·Journal of Biomedicine & Biotechnology·Xinghong YangDavid W Pascual
Dec 14, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Zhiyong SuoRecep Avci

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Citations

Oct 12, 2012·PloS One·Xinghong YangDavid W Pascual
Mar 15, 2014·Experimental & Molecular Medicine·David W PascualIrina Kochetkova
May 9, 2013·Virulence·David W PascualXinghong Yang
Jun 24, 2017·Clinical and Vaccine Immunology : CVI·Khandra T SearsMarcela F Pasetti
Jun 10, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Sukriye Celikkol-AydinRecep Avci

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